Nucleus accumbens dopamine and mu-opioid receptors modulate the reinstatement of food-seeking behavior by food-associated cues
Nucleus accumbens dopamine and mu-opioid receptors modulate the reinstatement of food-seeking behavior by food-associated cues
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DOI：
10.1016/j.bbr.2011.01.024
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发表时间：
2011-06-01
影响因子：
2.7
通讯作者：
Pratt, Wayne E.
中科院分区：
文献类型：
--
作者：
Guy, Elizabeth G.;Choi, Eugene;Pratt, Wayne E.
旨在促进健康体重的饮食干预的高损耗率可能是由持续暴露于与美味食物相关的环境刺激引起的，至少部分原因是。在人类和动物中，条件刺激（CSs）信号的奖励可用性可靠地恢复食物和药物寻求行为。伏隔核（NAcc）在线索诱发的寻找食物的恢复中起着至关重要的作用，但个体神经递质系统在NAcc中的作用仍有待确定。这些实验测试了伏核内多巴胺（DA） D-1和D-2受体、mu-阿片受体或5-羟色胺（5-HT）受体的药理操作对线索诱发的寻找食物复发的影响。训练大鼠杠杆按压蔗糖颗粒并同时呈现浅色CS。一旦训练完成，在没有蔗糖或CS的情况下，杠杆按压就会消失。一旦每只大鼠达到灭绝标准，它们接受两次恢复会话，其中通过反应条件下的CS呈现来更新杠杆按压。在每次恢复试验之前，大鼠接受NAcc微输注生理盐水或选择性D-1受体拮抗剂SCH 23390、D-2受体拮抗剂raclopride、mu-阿片受体激动剂[D-Ala2， N-MePhe4， Gly-ol]-脑啡肽（DAMGO）或5-HT马来酸氢。与生理盐水试验日相比，伏隔核内注射SCH 23390 （1 μ g/0.5 μ L）、raclopride （1 μ g/0.5 μ L）或DAMGO （0.25 μ g/0.5 μ L）有效阻断了线索诱发的寻找食物的恢复。相比之下，5-羟色胺（5- ht）受体被5-羟色胺马来酸氢（5 μ g/0.5 μ l）刺激对提示诱导的恢复没有影响。这些新数据支持NAcc DA D-1， D-2和mu-阿片受体在线索诱发的食物寻找恢复中的作用。(C) 2011 Elsevier B.V.版权所有
The high attrition rates for dietary interventions aimed at promoting a healthier body mass may be caused, at least in part, by constant exposure to environmental stimuli that are associated with palatable foods. In both humans and animals, conditioned stimuli (CSs) that signal reward availability reliably reinstate food- and drug-seeking behaviors. The nucleus accumbens (NAcc) is critically involved in the cue-evoked reinstatement of food-seeking, but the role of individual neurotransmitter systems within the NAcc remains to be determined. These experiments tested the effects of intra-accumbal pharmacological manipulations of dopamine (DA) D-1 and D-2 receptors, mu-opioid receptors, or serotonin (5-HT) receptors on cue-evoked relapse to food-seeking. Rats were trained to lever press for sucrose pellets and the concurrent presentation of a light-tone CS. Once training was complete, lever-pressing was extinguished in the absence of either sucrose or CS presentation. Once each rat had reached extinction criterion, they received two reinstatement sessions in which lever pressing was renewed by response-contingent presentation of the CS. Prior to each reinstatement test, rats received NAcc microinfusions of saline or the selective D-1 receptor antagonist SCH 23390, the D-2 receptor antagonist raclopride, the mu-opioid receptor agonist [D-Ala2, N-MePhe4, Gly-ol]-enkephalin (DAMGO), or 5-HT hydrogen maleate. Compared to saline test days, intra-accumbens infusions of SCH 23390 (1 mu g/0.5 mu L), raclopride (1 mu g/0.5 mu L), or DAMGO (0.25 mu g/0.5 mu L) effectively blocked the cue-evoked reinstatement of food-seeking. In contrast, stimulation of serotonin (5-HT) receptors by 5-HT hydrogen maleate (5 mu g/0.5 mu L.) had no effect on cue-induced reinstatement. These novel data support roles for NAcc DA D-1, D-2, and mu-opioid receptors in the cue-evoked reinstatement of food seeking. (C) 2011 Elsevier B.V. All rights reserved.