SUB-MEMBRANE RETICULUM OF HUMAN ERYTHROCYTE - SCANNING ELECTRON-MICROSCOPE STUDY
SUB-MEMBRANE RETICULUM OF HUMAN ERYTHROCYTE - SCANNING ELECTRON-MICROSCOPE STUDY
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DOI：
10.1002/jss.400060303
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
STECK, TL
中科院分区：
文献类型：
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作者：
HAINFELD, JF;STECK, TL
本文用扫描电子显微镜研究了人红细胞膜下的网状网，分辨率为5-10 nm。该网络可视化分离膜（鬼）撕开，揭示其内部空间和残留物中提取的鬼与Triton X-100。它在整个细胞质表面上形成连续的（而不是片状的）覆盖物，除了被剥离或撕裂的地方。纤维直径为5-40 nm，环状结构直径为40-60 nm，节点直径为30-100 nm。网中细丝和间隙的尺寸随条件而变化，表明网络具有弹性。这种网状物可能与红细胞膜蛋白血影蛋白和肌动蛋白有关。
A web-like reticulum underlying the human erythrocyte membrane was studied at a resolution of 5-10 nm using a scanning electron microscope. The network was visualized in isolated membranes (ghosts) torn open to reveal their interior space and in residues derived from ghosts extracted with Triton X-100. It formed a continuous (rather than patchy) cover over the entire cytoplasmic surface, except where lifted off or torn away. Filaments (5-40 nm in diameter), annular figures (40-60 nm in diameter) and nodes (30-100 nm in diameter) were prominent in different networks. The dimensions of the filaments and the interstices in the reticulum varied with conditions, suggesting that the network has elastic properties. This reticulum is probably related to the erythrocyte membrane proteins spectrin and actin.