U2 REGION OF EPSTEIN-BARR VIRUS-DNA MAY ENCODE EPSTEIN-BARR NUCLEAR ANTIGEN-2
U2 REGION OF EPSTEIN-BARR VIRUS-DNA MAY ENCODE EPSTEIN-BARR NUCLEAR ANTIGEN-2
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DOI：
10.1073/pnas.81.23.7632
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
KIEFF, E
中科院分区：
文献类型：
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作者：
DAMBAUGH, T;HENNESSY, K;KIEFF, E
对B 95 -8和AG 876 EB病毒（EBV）分离株的U2区的序列分析显示，在一个长的开放阅读框内存在分歧，该开放阅读框先前被鉴定为编码在潜伏感染的生长转化的B淋巴细胞系中表达的病毒RNA的3“末端的1.5个内切酶。U2开放阅读框架内的EBV分离株之间的差异与EBV核抗原EBNA 2的差异相关。B 95 -8、W 91、Raji、Cherry和拉蒙EBV分离株具有相似的U2多聚酶并编码相似大小的EBNA 2蛋白，而AG 876、Jijove和P3 HR-1具有变异或缺失的U2多聚酶和变异或缺失的EBNA 2蛋白。AG 876的U2开放阅读框和EBNA 2蛋白均短于B 95 -8。U2开放阅读框架可能编码EBNA 2。
Sequence analysis of the U2 regions of the B95-8 and AG876 Epstein-Barr virus (EBV) isolates reveals divergence within a long open reading frame previously identified as encoding 1.5 kilobases of the 3'' end of a viral RNA expressed in latently infected, growth-transformed, B-lymphocyte cell lines. Differences among EBV isolates within the U2 open reading frame correlate with differences in an EBV nuclear antigen, EBNA2. B95-8, W91, Raji, Cherry and Lamont EBV isolates have similar U2 doamins and encode similar-size EBNA2 proteins, while AG876, Jijove and P3HR-1 have variant or absent U2 doamins and variant or absent EBNA2 proteins. The AG876 U2 open reading frame and EBNA2 protein are both shorter than those of B95-8. The U2 open reading frame probably encodes EBNA2.