TRANSITION IN THE THIN-FILAMENT ARRANGEMENT IN RAT SKELETAL-MUSCLE
TRANSITION IN THE THIN-FILAMENT ARRANGEMENT IN RAT SKELETAL-MUSCLE
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DOI：
10.1007/bf00712001
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发表时间：
1983-01-01
影响因子：
2.7
通讯作者：
GOLDSTEIN, MA
中科院分区：
文献类型：
--
作者：
TRAEGER, L;MACKENZIE, JM;GOLDSTEIN, MA
用计算机辅助分析大鼠肌肉连续横截面的细丝排列，研究了细丝排列从Z带附近的四边形到A带的三角形的转变。从成年大鼠和成年，9天和3天的新生比目鱼肌（慢）肌的趾长伸肌（EDL;快）肌肉连续横切从H区的1个肌节的H区相邻的肌节。在整个I-带中分析细丝排列，特别是在肌节的3个水平处：在I-带中，1个区段（0.06 μ m）来自Z-带; 6个区段（0.36 μ m）来自Z-带;在A-带中，2个区段来自A-I接合处（0.72 μ m来自Z-带）。通过计算机获得了径向分布和环形分布的数据。在所有的肌肉研究中，细丝排列在整个I带中表现出4-邻居排序。细丝排列仅在A带表现出3邻有序。在静止长度固定的肌肉中，细丝排列从4-相邻排列到3-相邻排列的转变发生在A-I连接处的0.12 μ m内。在已经被拉伸20%使得A-I连接远离N2线的成年比目鱼肌中，在距离Z带0.4-0.5 μ m的区域内的I带中发生细丝排列的转变。该区域对应于I带的N2线区域。从4-邻居到3-邻居的转变发生在I带独立的薄磁-厚丝相互作用。一些固有的特征的I-带或薄的磁共振薄丝相互作用强加了4-邻居从Z-带的N2线的薄丝上的排序。
The transition in thin-filament arrangement from tetragonal near the Z-band to trigonal in the A-band was investigated by computer-assisted analysis of thin-filament arrangement in serially cross-sectioned rat muscle. Extensor digitorum longus (EDL; fast) muscle from adult rats and adult, 9-day and 3-day neonatal soleus (slow) muscle were serially cross-sectioned from the H-zone of 1 sarcomere to the H-zone of an adjacent sarcomere. Thin-filament arrangement was analyzed throughout the I-band, and particularly at 3 levels of the sarcomere: in the I-band, 1 section (0.06 .mu.m) from the Z-bank; 6 sections (0.36 .mu.m) from the Z-band; in the A-band, 2 sections from the A-I junction (0.72 .mu.m from the Z-band). Data for radial distributions and annular distributions were obtained by computer. In all muscle studies, thin-filament arrangement exhibited 4-neighbor ordering throughout the I-band. Thin-filament arrangement exhibited 3-neighbor ordering only in the A-band. The transition in thin-filament arrangement from 4-neighbor to 3-neighbor ordering occurred within 0.12 .mu.m of the A-I junction in muscles fixed at rest length. In adult soleus that had been stretched 20% so that the A-I junction moved away from the N2-line, the transition in thin-filament arrangement occurred in the I-band within a region 0.4-0.5 .mu.m from the Z-band. This region corresponds to the N2-line region of the I-band. The transition from 4-neighbor to 3-neighbor ordering occurs in the I-band independent of the thin filament-thick filament interaction. Some inherent feature of the I-band or thin filament-thin filament interaction imposes a 4-neighbor ordering on thin filaments from the Z-band to the N2-line.