Amino acid and adenine cross-pathway regulation act through the same 5'-TGACTC-3' motif in the yeast HIS7 promoter
Amino acid and adenine cross-pathway regulation act through the same 5'-TGACTC-3' motif in the yeast HIS7 promoter
复制标题
DOI：
10.1074/jbc.271.47.29637
复制
发表时间：
1996-11-22
影响因子：
4.8
通讯作者：
Braus, GH
中科院分区：
文献类型：
--
作者：
Springer, C;Kunzler, M;Braus, GH
酿酒酵母的HIS7基因编码双功能谷氨酰胺转移酶：环化酶，催化两个反应，导致氨基酸组氨酸和嘌呤腺嘌呤的生物合成中间体的形成。HIS7基因在氨基酸饥饿的环境条件下通过两个协同上游位点GCRE1和GCRE2被GCN 4p激活。BAS1p-BAS2p复合物响应腺嘌呤限制而激活HIS 7基因。对于这种激活，需要近端GCN 4p结合位点GCRE2。GCN 4p和BAS 1p在体外与GCRE2结合。在同时氨基酸饥饿和腺嘌呤限制的条件下，GCN 4p和BAS 1/2p的作用是加和的，并且这两个因子对于最大化HIS 7转录是必需的。这些结果表明，GCN 4p和BAS 1/2p能够在体内通过相同的DNA序列同时起作用，并且以非排他性的方式彼此独立地使用该位点。
The HIS7 gene of Saccharomyces cerevisiae encodes a bifunctional glutamine amidotransferase:cyclase catalyzing two reactions that lead to the formation of biosynthetic intermediates of the amino acid histidine and the purine adenine. The HIS7 gene is activated by GCN4p under environmental conditions of amino acid starvation through two synergistic upstream sites GCRE1 and GCRE2. The BAS1p-BAS2p complex activates the HIS7 gene in response to adenine limitation. For this activation the proximal GCN4p-binding site GCRE2 is required. GCN4p and BAS1p bind to GCRE2 in vitro. Under conditions of simultaneous amino acid starvation and adenine limitation the effects of GCN4p and BAS1/2p are additive and both factors are necessary for maximal HIS7 transcription. These results suggest that GCN4p and BAS1/2p are able to act simultaneously through the same DNA sequence in vivo and use this site independently from each other in a non-exclusive manner.