Altered RNA/protein ratio associated with the induction of differentiation of Friend erythroleukemia cells.
Altered RNA/protein ratio associated with the induction of differentiation of Friend erythroleukemia cells.
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RNA/蛋白质比例的改变与 Friend 红白血病细胞分化的诱导相关。
DOI：
10.1073/pnas.78.6.3882
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发表时间：
1981
影响因子：
11.1
通讯作者：
Friend,C
中科院分区：
文献类型：
--
作者：
Harel,L;Blat,C;Lacour,F;Friend,C
本文研究了用氨基核苷嘌呤霉素(AMS)诱导分化的Friend红白血病(FL)细胞和加入肌苷抑制分化的FL细胞中RNA的合成和积累。当FL细胞在AMS存在的情况下生长时，RNA合成被显著抑制。这种效应不能完全归因于对从头合成嘌呤的抑制，因为从标记的肌苷生物合成嘌呤核苷酸的抑制要小得多。与未经处理的对照组相比，ATP/蛋白质和GTP/蛋白质的比率略有改变。但RNA/蛋白质比值降低。因此，细胞的RNA含量降低了30%-40%，但蛋白质含量没有受到明显影响。当AMS与肌苷共同作用于细胞时，当AMS与肌苷一起抑制AMS诱导的分化时，与单独用AMS处理的细胞相比，RNA/蛋白质比率增加，并接近未处理的对照细胞的水平。腺苷在克服AMS对RNA合成的抑制方面具有类似的作用。由于另外两种有效的诱导剂二甲基亚砜或丁酸钠处理FL细胞后，细胞的RNA/蛋白质比下降了44%，我们的结果表明细胞的RNA含量与诱导剂的诱导分化之间存在相关性。
The synthesis and accumulation of RNA in Friend erythroleukemia (FL) cells induced to differentiate by treatment with the aminonucleoside of puromycin (AMS) or inhibited from differentiating by the addition of inosine to the medium were studied. When FL cells were grown in the presence of AMS, RNA synthesis was substantially inhibited. This effect could not be attributed solely to the inhibition of de novo purine synthesis because the biosynthesis of purine nucleotides from labeled inosine was much less depressed. The ratios of ATP to protein and of GTP to protein were slightly modified as compared to the untreated controls. However, the RNA/protein ratio was decreased. Thus, the RNA content of the cells was reduced 30-40%, but the protein content was not significantly affected. When the cells were treated with AMS together with inosine at a concentration that inhibits AMS-induced differentiation, the RNA/protein ratio was increased as compared with that found in cells treated with AMS alone and approached the level of the ratio in untreated control cells. Adenosine had a similar effect in overcoming the inhibition of RNA synthesis by AMS. Because the RNA/protein ratio of FL cells treated with dimethyl sulfoxide or sodium butyrate, two other potent inducers, was decreased by 44%, our results suggest that a correlation exists between the RNA content of the cells and the triggering of differentiation by inducers.