Bioactive surface modification of mica and poly(dimethylsiloxane) with hydrophobins for protein immobilization
Bioactive surface modification of mica and poly(dimethylsiloxane) with hydrophobins for protein immobilization
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DOI：
10.1021/la062744h
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发表时间：
2007-04-10
期刊：
影响因子：
3.9
通讯作者：
Qiao, Ming-Qiang
中科院分区：
文献类型：
--
作者：
Qin, Ming;Wang, Li-Kai;Qiao, Ming-Qiang
疏水蛋白II（HFBI）在云母和聚二甲基硅氧烷（PDMS）表面的自组装可以获得具有适当亲水性的生物活性表面，用于蛋白质固定。X射线光电子能谱和水接触角的测量表明，表面润湿性可以从超疏水（PDMS）或超亲水（云母）到适度亲水，这是适合于蛋白质（鸡IgG）固定在两个基板表面。研究结果表明，HFBI组装体是一种通用、方便的生物分子固定化方法，在生物传感器、免疫分析和微流控网络等领域具有潜在的应用前景。
Bioactive surfaces with appropriate hydrophilicity for protein immobilization can be achieved by hydrophobin II (HFBI) self-assembly on mica and polydimethylsiloxane (PDMS) surfaces. X-ray photoelectron spectroscopy and water contact angle measurements illustrated that the surface wettability can be changed from superhydrophobic (PDMS) or superhydrophilic (mica) to moderately hydrophilic, which is suitable for protein (chicken IgG) immobilization on both substrate surfaces. The results suggest that HFBI assembly, one kind of hydrophobin from Trichoderma reesei, may be a versatile and convenient method for the immobilization of biomolecules on diverse substrates, which may have potential applications in biosensors, immunoassays, and microfluidic networks.