Protection against polycyclic aromatic hydrocarbon-induced skin tumor initiation in mice by green tea polyphenols.
Protection against polycyclic aromatic hydrocarbon-induced skin tumor initiation in mice by green tea polyphenols.
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DOI：
10.1093/carcin/10.2.411
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发表时间：
1989-02
期刊：
影响因子：
4.7
通讯作者：
Z. Y. Wang;Wasiuddin A. Khan;D. Bickers;Hasan Mukhtar
中科院分区：
文献类型：
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作者：
Z. Y. Wang;Wasiuddin A. Khan;D. Bickers;Hasan Mukhtar
绿茶在中国和亚洲是一种很受欢迎的饮料，已经被证明具有解热、利尿和其他几种药理活性。绿茶的主要成分是具有抗氧化和抗诱变特性的多酚。研究了绿茶多酚（GTP）对多环芳烃（PAHs）致小鼠皮肤肿瘤的抑制作用。在使用3-甲基胆蒽的完整皮肤肿瘤发生方案中，GTP局部应用于雌性BALB/c小鼠，对肿瘤的发生和随后的发展产生了实质性的保护作用。在两阶段的皮肤肿瘤发生方案中，使用7,12-二甲基苯[a]蒽（DMBA）作为引发剂，随后每周两次应用12- o-十四烷基酚-13-乙酸酯；（TPA）作为肿瘤促进剂，局部应用GTP对雌性senar小鼠的皮肤致瘤性有显著的保护作用。口服饮用水中的GTP对雌性senar小鼠也有保护作用，可以防止DMBA - tpa处理动物的皮肤肿瘤发生。局部或口服GTP可显著抑制局部应用[3H]苯并[a]芘或[3H]DMBA后表皮中PAH - DNA加合物的形成。我们的研究结果表明，GTP对多环芳烃具有实质性的抗皮肤肿瘤启动活性，并且可以证明对某些形式的人类癌症有保护作用。
Green tea is a popular beverage in China and Asia and has been shown to possess antipyretic, diuretic and several other pharmacological activities. The major constituents of green tea are polyphenols which have been found to possess antioxidant and antimutagenic properties. In this study green tea polyphenols (GTP) were evaluated as an anti-initiating agent against the skin tumorigenicity induced by polycyclic aromatic hydrocarbons (PAHs) in mice. In a complete skin tumorigenesis protocol using 3-methylcholanthrene the topical application of GTP to female BALB/c mice resulted in substantial protection against the onset and subsequent development of tumors. In the two-stage skin tumorigenesis protocol using 7,12-dimethylbenz[a]anthracene (DMBA) as the initiating agent followed by twice weekly applications of 12-O-tetradecanoylphorbol-13-acetate; (TPA) as tumor promoter, topical application of GTP to female SENCAR mice afforded significant protection against skin tumorigenicity. Oral feeding of GTP in drinking water to female SENCAR mice also protected against skin tumorigenesis in DMBA - TPA-treated animals. GTP when administered topically or orally significantly inhibited PAH - DNA adduct formation in epidermis after topical application of [3H]benzo[a]pyrene or [3H]DMBA. Our results suggest that GTP has substantial anti-skin-tumor-initiating activity against PAHs and could prove useful in protecting against some forms of human cancer.