Effect of polymer chemistry and fabrication method on protein release and stability from polyanhydride microspheres.
Effect of polymer chemistry and fabrication method on protein release and stability from polyanhydride microspheres.
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DOI：
10.1002/jbm.b.31478
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发表时间：
2009-11
影响因子：
3.4
通讯作者：
Narasimhan, Balaji
中科院分区：
文献类型：
--
作者：
Lopac, Senja K.;Torres, Maria P.;Wilson-Welder, Jennifer H.;Wannemuehler, Michael J.;Narasimhan, Balaji
研究了不同化学组成的聚酸酐微球包封卵清蛋白的释放动力学和稳定性。使用基于酸酐单体癸二酸（SA）、1，6-双（对羧基苯氧基）己烷（CPH）和1，8-双（对羧基苯氧基）-3，6-二氧杂辛烷（CPTEG）的聚合物。使用两种非水方法制备微球：固体/油/油双乳液技术和低温雾化。研究表明，这两种制造方法没有显着影响卵清蛋白的释放动力学，即使蛋白质的爆发释放是制造方法和聚合物化学的函数。采用免疫印迹法研究了冷冻雾化法制备的卵清蛋白微球的抗原稳定性。这些研究表明，两亲性CPTEG：CPH聚酐通过保留释放蛋白的免疫表位来保持蛋白质结构并增强蛋白质稳定性。
The release kinetics and stability of ovalbumin encapsulated into polyanhydride microspheres with varying chemistries were studied. Polymers based on the anhydride monomers sebacic acid (SA), 1,6-bis(p-carboxyphenoxy)hexane (CPH), and 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) were utilized. Microspheres were fabricated using two non-aqueous methods: a solid/oil/oil double emulsion technique and cryogenic atomization. The studies showed that the two fabrication methods did not significantly affect the release kinetics of ovalbumin, even though the burst release of the protein was a function of the fabrication method and the polymer chemistry. Antigenic stability of ovalbumin released from microspheres prepared by cryogenic atomization was studied by western blot analysis. These studies indicate that the amphiphilic CPTEG:CPH polyanhydrides preserved protein structure and enhanced protein stability by preserving the immunological epitopes of released protein.
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影响因子：
3.7
作者：
COSTANTINO, HR;LANGER, R;KLIBANOV, AM
通讯作者：
KLIBANOV, AM
影响因子：
14
作者：
Determan, AS;Wilson, JH;Narasimhan, B
通讯作者：
Narasimhan, B
影响因子：
3.7
作者：
Cleland, JL;Jones, AJS
通讯作者：
Jones, AJS
DOI：
10.1002/jbm.820190806
发表时间：
1985-01-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
LEONG, KW;BROTT, BC;LANGER, R
通讯作者：
LANGER, R
影响因子：
10.8
作者：
Determan, AS;Trewyn, BG;Narasimhan, B
通讯作者：
Narasimhan, B