Absence of catalase reduces long-term survival of Helicobacter pylori in macrophage phagosomes
Absence of catalase reduces long-term survival of Helicobacter pylori in macrophage phagosomes
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DOI：
10.1111/j.1083-4389.2004.00226.x
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发表时间：
2004-06-01
期刊：
影响因子：
4.4
通讯作者：
Blanchard, TG
中科院分区：
文献类型：
--
作者：
Basu, M;Czinn, SJ;Blanchard, TG
背景。一些幽门螺杆菌菌株可以在巨噬细胞吞噬体内存活长达 24 小时。影响这种生存的因素仍然不明确。因此，在体外研究了过氧化氢酶在介导幽门螺杆菌吞噬后存活的作用。方法。产生了同基因的、过氧化氢酶缺陷的幽门螺杆菌菌株，并测试了其对过氧化氢的敏感性和对巨噬细胞介导的杀伤的敏感性。结果。与保持 100% 存活长达 21 分钟的野生型幽门螺杆菌相比，过氧化氢可在 3 分钟内有效杀死等基因、过氧化氢酶缺陷型幽门螺杆菌菌株。即使细菌与巨噬细胞的比例增加，过氧化氢酶缺陷突变体也比亲本菌株更容易受到巨噬细胞介导的杀伤。结论。这些结果表明，虽然幽门螺杆菌的一些菌株能够在巨噬细胞吞噬体内存活，但存活依赖于毒力因子，例如用于逃避先天宿主防御的过氧化氢酶。
Background. Some Helicobacter pylori strains can survive within macrophage phagosomes for up to 24 hours. The factors that play a role in this survival remain ill-defined. Therefore, the contribution of catalase in mediating the survival of H. pylori following phagocytosis was investigated in vitro.Methods. An isogenic, catalase-deficient strain of H. pylori was generated and tested for sensitivity to hydrogen peroxide and susceptibility to macrophage-mediated killing.Results. The isogenic, catalase-deficient strain of H. pylori was effectively killed by hydrogen peroxide within 3 minutes compared to wild-type H. pylori which maintained 100% survival up to 21 minutes. The catalase-deficient mutant was also significantly more susceptible to macrophage-mediated killing than the parent strain, even when the ratio of bacteria to macrophage was increased.Conclusion. These results indicate that although some strains of H. pylori are capable of survival within the macrophage phagosome, survival is dependent on virulence factors such as catalase for evasion of innate host defense.