In vitro effects of enamel matrix proteins on rat bone marrow cells and gingival fibroblasts
In vitro effects of enamel matrix proteins on rat bone marrow cells and gingival fibroblasts
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DOI：
10.1177/154405910408300210
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发表时间：
2004-02-01
影响因子：
7.6
通讯作者：
Weinreb, M
中科院分区：
文献类型：
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作者：
Keila, S;Nemcovsky, CE;Weinreb, M
Emdogain(R) （EMD）是牙釉质基质蛋白（EMP）的一种配方，临床上用于牙周再生，它可以刺激牙骨质形成并促进牙龈愈合。本实验研究了EMD对大鼠骨髓基质细胞（BMSC）和牙龈成纤维细胞（GF）的体外影响。EMD（25马克杯/毫升）增加了骨髓的成骨能力，BMSC细胞数量增加了3倍，碱性磷酸酶（ALP）活性增加了2倍，矿化结节形成也增加了2倍。EMD在培养的最初阶段（前48小时）的存在对这种效果至关重要。相比之下，EMD没有诱导GF的成骨细胞分化（缺乏矿化或ALP活性证明），但其数量和基质产生量增加了两倍。这些对BMSC和GF的体外实验数据可以解释EMD对骨形成和结缔组织再生的促进作用。
Emdogain(R) (EMD), a formulation of Enamel Matrix Proteins (EMP), is used clinically for periodontal regeneration, where it stimulates cementum formation and promotes gingival healing. In this study, we investigated the in vitro effects of EMD on rat bone marrow stromal cells (BMSC) and gingival fibroblasts (GF). EMD (at 25 mug/mL) increased the osteogenic capacity of bone marrow, as evidenced by similar to three-fold increase in BMSC cell number and similar to two-fold increase in alkaline phosphatase (ALP) activity and mineralized nodule formation. The presence of EMD in the initial stages (first 48 hrs) of the culture was crucial for this effect. In contrast, EMD did not induce osteoblastic differentiation of GF (evidenced by lack of mineralization or ALP activity) but increased up to two-fold both their number and the amount of matrix produced. These in vitro data on BMSC and GF could explain the promotive effect of EMD on bone formation and connective tissue regeneration, respectively.