Detection of synthetic RGDS (PO3H2) PA peptide adsorption using a titanium surface plasmon resonance (SPR) biosensor
Detection of synthetic RGDS (PO3H2) PA peptide adsorption using a titanium surface plasmon resonance (SPR) biosensor
复制标题
使用钛表面等离子共振 (SPR) 生物传感器检测合成 RGDS (PO3H2) PA 肽吸附
DOI：
10.1007/s10856-010-4222-2
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Abe;Y.;Hiasa;K.;Hirata;I.;Okazaki;Y.;Nogami;K.;Mizumachi;W.;Yoshida;Y.;Suzuki;K.;Okazaki;M.;Akagawa;Y.
本研究的目的是测量合成的RGDS（PO 3 H2）PA（P-RGD）肽和钛表面之间的时间依赖性的化学相互作用，使用钛表面等离子体共振（SPR）生物传感器，并确定肽固定在表面上的程度。根据Knoll方法，使用用于“单点”分析的SPR仪器，使用He-Ne激光对生物分子吸附进行纳米级检测。当暴露于pH为2.0或更低的H3 PO 4溶液时，氧化的钛表面被蚀刻。pH1.9时P-RGD的吸附量是pH3.0时的3.6倍（P< 0.05）。P-RGD自然吸附在钛氧化物表面上作为磷酸盐官能团的键合和解离机制的结果。此外，pH值的控制对P-RGD与表面的相互作用起着非常重要的作用。这些发现表明，pH控制可以促进具有磷酸盐官能团的生物分子与钛表面的渐进结合。
The purpose of this study was to measure the time-dependent chemical interaction between synthetic RGDS(PO3H2)PA (P-RGD) peptide and titanium surfaces using a titanium surface plasmon resonance (SPR) biosensor and to determine the degree of peptide immobilization on the surfaces. An SPR instrument for ‘single-spot’ analysis was used for nanometer-scale detection of biomolecular adsorption using a He–Ne laser light according to Knoll’s method. The oxidized titanium surface was etched when exposed to H3PO4solutions with a pH of 2.0 or below. The amount of P-RGD adsorbed at pH 1.9 was approximately 3.6 times as much as that at pH 3.0 (P< 0.05). P-RGD naturally adsorbed on the oxidized titanium surface as a consequence of the bonding and dissociation mechanism of the phosphate functional group. Furthermore, the control of pH played a very important role in the interaction between P-RGD and the surface. These findings show that pH control may promote progressive binding of biomolecules with the phosphate functional group to the titanium surface.