Let‐7f Inhibits Glioma Cell Proliferation, Migration, and Invasion by Targeting Periostin
Let‐7f Inhibits Glioma Cell Proliferation, Migration, and Invasion by Targeting Periostin
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DOI：
10.1002/jcb.25128
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发表时间：
2015-08
影响因子：
4
通讯作者：
Shaofeng Yan;Xiao Han;H. Xue;Ping Zhang;Xing Guo;Tong Li;Xiaofan Guo;G. Yuan;Lin Deng;Gang Li
中科院分区：
文献类型：
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作者：
Shaofeng Yan;Xiao Han;H. Xue;Ping Zhang;Xing Guo;Tong Li;Xiaofan Guo;G. Yuan;Lin Deng;Gang Li
胶质瘤是最具侵袭性和恶性的肿瘤类型之一。尽管在手术、影像学、化疗和放疗方面取得了进展，但胶质瘤患者的预后仍然很差。脑胶质瘤的发病机制是一个亟待解决的问题。microRNA（miRNAs）是内源性小的非编码RNA，是基因表达的关键转录后调节因子。miRNA失调通常发生在人类肿瘤发生中。在本研究中，Let-7 f的表达水平在胶质瘤组织和胶质瘤细胞中均下调。Let-7 f的增强表达通过直接靶向perisotin致癌活性抑制胶质瘤细胞增殖、迁移和侵袭。骨膜蛋白siRNA或过表达的实验进一步表明，Let-7 f可以通过perisotin信号作为肿瘤抑制剂。这些发现为Let-7 f通过Let-7 f/periostin轴调节胶质瘤细胞生物学行为的作用和机制提供了新的见解，并且Let-7 f可能作为胶质瘤的潜在治疗靶点。J.细胞。116：1680-1692，2015.© 2015威利期刊公司.
Glioma is one of the most aggressive and malignant tumor types. Despite advances in surgery, imaging, chemotherapy, and radiation, glioma patient prognosis remains poor. Glioma pathogenesis is an urgent problem that must be solved. MicroRNAs (miRNAs) are endogenous small non‐coding RNAs that are key post‐transcriptional regulators of gene expression. miRNA deregulation commonly occurs in human tumorigenesis. In the present study, the expression levels of Let‐7f were down‐regulated in both glioma tissues and glioma cells. The enhanced expression of Let‐7f suppressed glioma cells proliferation, migration, and invasion via direct targeting perisotin oncogenic activity. Experiments with periostin siRNA or over‐expression further suggest that Let‐7f may serve as tumor suppressors through perisotin signal. These findings provide insights regarding the role and mechanism of Let‐7f in regulating biological behavior of glioma cells via the Let‐7f/periostin axis, and Let‐7f may serve as a potential therapeutic target in glioma. J. Cell. Biochem. 116: 1680–1692, 2015. © 2015 Wiley Periodicals, Inc.