Electron microprobe analysis of intracellular elements in the rat kidney.
Electron microprobe analysis of intracellular elements in the rat kidney.
复制标题
大鼠肾脏细胞内元素的电子探针分析。
作者：
F. Beck;Richard Bauer;Ulrike Bauer;June Mason;A. Dörge;R. Rick;Klaus Thurau
细胞内元素的浓度测定通过电子探针分析在细胞核和细胞质的冷冻干燥冷冻切片的表面近端和远端小管的大鼠肾脏。对于近端小管细胞的细胞核，钠和氯的浓度为20和23毫摩尔/千克湿重，钾和磷的浓度为144和150毫摩尔/千克湿重。对于远端肾小管细胞的细胞核，钠和氯的浓度显著降低（11和13毫摩尔/千克湿重），钾的浓度不变（143毫摩尔），磷的浓度显著升高（175毫摩尔）。朝向近端和远端小管的基底外侧内折和近端小管的刷状缘，钠和氯的浓度较高，钾和磷的浓度低于在细胞核中获得的浓度，表明在这些区域存在细胞外室。在近端和远端肾小管细胞靠近细胞核的中央细胞质中进行的测量显示，钠和钾浓度与细胞核中的浓度难以区分，而氯和磷浓度则相当高。这些数据表明，在近端和远端肾小管细胞之间的磷，钠和氯的细胞内浓度的差异。然而，无论是在近端还是远端肾小管细胞中，细胞核和细胞质之间的钠和钾的浓度差都不能确定。
The concentrations of intracellular elements were determined by electron microprobe analysis in the nucleus and cytoplasm of freeze-dried cryosections of superficial proximal and distal tubules of the rat kidney. For the nucleus of the proximal tubular cell, the concentrations of sodium and chloride were 20 and 23 mmoles/kg of wet wt, and those of potassium and phosphorus were 144 and 150 mmoles/kg wet wt. For the nucleus of the distal tubular cell, the concentrations of sodium and chloride were significanlty lower (11 and 13 mmoles/kg wet wt), that of potassium was unchanged (143 mmoles), and that of phosphorus was significantly higher (175 mmoles). Towards the basolateral infoldings of the proximal and distal tubules and the brush border of the proximal tubules, the concentrations of sodium and chloride were higher and those of potassium and phosphorus were lower than those obtained in the nucleus, indicating the presence of extracellular compartments in these regions. Measurements performed in the centrally located cytoplasm of proximal and distal tubular cells, close to the nucleus, showed sodium and potassium concentrations to the indistinguishable from those in the nucleus, whereas chloride and phosphorus concentrations were considerably higher. These data demonstrate differences in the intracellular concentrations of phosphorus, sodium, and chloride between proximal and distal tubular cells. In neither the proximal nor the distal tubular cells, however, could a concentration difference for sodium and potassium between nucleus and cytoplasm be determined.