Merit of Astragalus polysaccharide in the improvement of early diabetic nephropathy with an effect on mRNA expressions of NF-κB and IκB in renal cortex of streptozotoxin-induced diabetic rats
Merit of Astragalus polysaccharide in the improvement of early diabetic nephropathy with an effect on mRNA expressions of NF-κB and IκB in renal cortex of streptozotoxin-induced diabetic rats
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DOI：
10.1016/j.jep.2007.08.024
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发表时间：
2007-12-03
影响因子：
5.4
通讯作者：
Cheng, Hei-Tang
中科院分区：
文献类型：
--
作者：
Zhang, Ying-Wen;Wu, Chao-Yan;Cheng, Hei-Tang
研究目的：糖尿病肾病（Diabetic nephropathy， DN）是导致终末期肾功能衰竭的主要原因，但目前预防糖尿病肾病的策略效果仍然较差。转录因子核因子κ B （nf - κ B）的激活被认为是DN发病的关键步骤。本研究研究了黄芪多糖（Astragalus多糖，APS）对链脲霉素诱导的实验性DN雄性sd大鼠NF-kappa B基因表达和核因子-kappa B抑制蛋白（I kappa B）的影响。材料与方法：以血管紧张素转换酶抑制剂黄芪多糖（1 g/kg p.o）或苯那普利（1.5 mg/kg p.o）治疗DN大鼠为阳性对照。测定血糖、血脂、微量白蛋白尿等生化指标。采用逆转录-聚合酶链反应测定肾皮质NF-kappa B或I -kappa B mRNA水平。结果：治疗8周后，黄芪多糖改善了患者无光泽、毛发刚毛、多尿、多饮、嗜睡、不活动、体重下降、后凸、卧位等症状。与对照大鼠相比，aps治疗大鼠的血糖、血脂和微量白蛋白尿水平均降低。经黄芪多糖治疗后，大鼠肾重/体重比降低，肾功能改善。黄芪多糖可降低肾皮质NF-kappa B mRNA表达，提高I -kappa B mRNA表达。提示黄芪多糖对DN的进展具有预防和治疗作用；结论：APS有助于早期预防和/或治疗DN。2007爱思唯尔爱尔兰有限公司版权所有。
Aim of study: Diabetic nephropathy (DN) is the leading cause of the end-stage failure of kidney, but the efficacy of currently available strategies for the prevention of DN remains poor. An activation of the transcription factor, nuclear factor-kappa B (NF-kappa B), has been suggested to be a key step in the pathogenesis of DN. In the present study, we investigated the effect of Astragalus polysaccharide (APS), an aqueous extract from the Astragalus membranaceus roots, on gene expressions of NF-kappa B and an inhibitory protein of nuclear factor-kappa B (I kappa B) in experimental DN induced by streptozotocin in male Sprague-Dawley rats.Materials and Method: Rats with DN were treated with APS (1 g/kg p.o.) or benazepril (1.5 mg/kg p.o.), an angiotensin-converting enzyme inhibitor, using as positive control. The biochemical parameters such as blood glucose, plasma lipid and microalbuminuria were measured. Also, the mRNA level of NF-kappa B or I kappa B in renal cortex was determined using reverse transcription-polymerase chain reaction.Results: Eight weeks after the treatment, symptoms including shineless, bristly hair, polyuria, polydipsia, lethargy, physical inactivity, loss of body weight, kyphosis and decubitus position were ameliorated by APS. The levels of blood glucose, plasma lipid and microalbuminuria were lowered in APS-treated rats compared with control rats. The ratio of kidney weight over body weight was reduced and the renal function was improved after APS treatment. The mRNA level of NF-kappa B in renal cortex was decreased and I kappa B mRNA expression was raised by APS. These results suggest that APS has prophylactic and therapeutic effects on the progress of DN;Conclusions: therefore, APS is helpful for the prevention and/or treatment of DN at early stage. (c) 2007 Elsevier Ireland Ltd. All rights reserved.