MOLECULAR CHARACTERIZATION OF THE 128-KDA IMMUNODOMINANT ANTIGEN OF HELICOBACTER-PYLORI-ASSOCIATED WITH CYTOTOXICITY AND DUODENAL-ULCER
MOLECULAR CHARACTERIZATION OF THE 128-KDA IMMUNODOMINANT ANTIGEN OF HELICOBACTER-PYLORI-ASSOCIATED WITH CYTOTOXICITY AND DUODENAL-ULCER
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DOI：
10.1073/pnas.90.12.5791
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发表时间：
1993-06-15
影响因子：
11.1
通讯作者：
RAPPUOLI, R
中科院分区：
文献类型：
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作者：
COVACCI, A;CENSINI, S;RAPPUOLI, R
幽门螺杆菌与胃炎、消化性溃疡和胃腺癌有关。我们报道了H.幽门螺杆菌和疾病期间对抗原的免疫应答。该抗原被命名为CagA（细胞毒素相关基因A），是由大多数临床分离株产生的128 kDa的亲水性表面暴露蛋白。cagA基因及其蛋白的大小在不同菌株中通过涉及基因内区域复制的机制而变化。不产生抗原的临床分离株不具有该基因，并且不能产生活性空泡化细胞毒素。检测针对该蛋白的重组片段的免疫应答的ELISA检测到75.3%的胃十二指肠疾病患者和100%的十二指肠溃疡患者（P < 0.0005），表明只有携带该蛋白的细菌与疾病相关。
Helicobacter pylori has been associated with gastritis, peptic ulcer, and gastric adenocarcinoma. We report the nucleotide sequence and expression of an immunodominant antigen of H. pylori and the immune response to the antigen during disease. The antigen, named CagA (cytotoxin-associated gene A), is a hydrophilic, surface-exposed protein of 128 kDa produced by most clinical isolates. The size of the cagA gene and its protein varies in different strains by a mechanism that involves duplication of regions within the gene. Clinical isolates that do not produce the antigen do not have the gene and are unable to produce an active vacuolating cytotoxin. An ELISA to detect the immune response against a recombinant fragment of this protein detects 75.3% of patients with gastroduodenal diseases and 100% of patients with duodenal ulcer (P < 0.0005), suggesting that only bacteria harboring this protein are associated with disease.