Intramyocellular lipid concentrations are correlated with insulin sensitivity in humans:: a 1H NMR spectroscopy study
Intramyocellular lipid concentrations are correlated with insulin sensitivity in humans:: a 1H NMR spectroscopy study
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DOI：
10.1007/s001250051123
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发表时间：
1999-01-01
期刊：
影响因子：
8.2
通讯作者：
Roden, M
中科院分区：
文献类型：
--
作者：
Krssak, M;Petersen, KF;Roden, M
最近的肌肉活检研究表明肌内脂质含量和胰岛素抵抗之间的关系。本研究的目的是测试这种关系在人类中使用一种新的质子核磁共振（H-1 NMR）光谱技术，使非侵入性和快速（类似于45分钟）测定肌细胞内脂质（IMCL）含量。采用横断面分析研究了正常体重非糖尿病成人（n = 23，年龄29 ± 2岁，BMI = 24.1 ± 0.5 kg/m2）。通过2小时高胰岛素血症（类似于450 pmol/l）-正常胰岛素血症（类似于5 mmol/l）钳夹试验评估胰岛素敏感性。肌细胞内脂质浓度测定使用本地化的H-1 NMR光谱的比目鱼肌。简单线性回归分析显示，肌细胞内脂质含量与M值（钳夹100-120 min）以及空腹血浆非酯化脂肪酸浓度与M值（r =-0.54，p = 0.0267）之间呈负相关（r =-0.692，p = 0.0017）。肌细胞内脂质含量与BMI、年龄和空腹血浆甘油三酯、非酯化脂肪酸、葡萄糖或胰岛素浓度无关。这些结果表明，如通过局部H-1 NMR光谱法非侵入性评估的肌细胞内脂质浓度是非糖尿病、非肥胖人的全身胰岛素敏感性的良好指标。
Recent muscle biopsy studies have shown a relation between intramuscular lipid content and insulin resistance. The aim of this study was to test this relation in humans by using a novel proton nuclear magnetic resonance (H-1 NMR) spectroscopy technique, which enables non-invasive and rapid (similar to 45 min) determination of intramyocellular lipid (IMCL) content. Normal weight non-diabetic adults (n = 23, age 29 +/- 2 years, BMI = 24.1 +/- 0.5 kg/m(2)) were studied using cross-sectional analysis. Insulin sensitivity was assessed by a 2-h hyperinsulinaemic (similar to 450 pmol/l)-euglycaemic (similar to 5 mmol/l) clamp test. Intramyocellular lipid concentrations were determined by using localized H-1 NMR spectroscopy of soleus muscle. Simple linear regression analysis showed an inverse correlation (r = -0.692, p = 0.0017) between intramyocellular lipid content and M-value (100-120 min of clamp) as well as between fasting plasma non-esterified fatty acid concentration and M-value (r = -0.54, p = 0.0267). Intramyocellular lipid content was not related to BMI, age and fasting plasma concentrations of triglycerides, non-esterified fatty acids, glucose or insulin. These results show that intramyocellular Lipid concentration, as assessed non invasively by localized H-1 NMR spectroscopy, is a good indicator of whole body insulin sensitivity in non-diabetic, non-obese humans.