Protective Effect of Sodium Ferulate on Acetaldehyde-Treated Precision-Cut Rat Liver Slices
Protective Effect of Sodium Ferulate on Acetaldehyde-Treated Precision-Cut Rat Liver Slices
复制标题
阿魏酸钠对乙醛处理的精密切割大鼠肝片的保护作用
DOI：
10.1089/jmf.2011.1915
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发表时间：
2012-06-01
影响因子：
2.4
通讯作者：
Wang, Hui
中科院分区：
文献类型：
--
作者：
Guo, Yu;Wu, Xiao-Qian;Wang, Hui
激活的肝星状细胞（HSC）在肝纤维化中起着关键作用，抑制HSC的激活可以预防肝纤维化。乙醛是酒精最有害的代谢物，可在酒精性肝损伤中引发HSC活化。在本研究中，我们利用精确切肝片（PCLSs）研究了阿魏酸钠（SF）对乙醛刺激的HSC活化的保护作用，阿魏酸钠是一种富含水果和蔬菜的阿魏酸钠盐。将大鼠pcls与700 μ M乙醛和不同浓度的SF共孵育。肝毒性通过测量组织中酶渗漏和丙二醛含量来评估。测定α -平滑肌肌动蛋白、转化生长因子- β(1)和羟脯氨酸来评估造血干细胞的活化。此外，测定基质金属蛋白酶(MMP)-1和组织金属蛋白酶抑制剂（TIMP-1）来评价胶原降解。SF显著地阻止了乙醛处理切片中的酶渗漏，并抑制了乙醛刺激的HSC活化和胶原蛋白的产生。此外，SF增加MMP-1表达，降低TIMP-1表达。上述结果表明，SF可保护pcls免受乙醛刺激的HSC活化和肝损伤，这可能与减弱氧化损伤和加速胶原降解有关。
Activated hepatic stellate cells (HSCs) play a key role in hepatic fibrogenesis, and inhibition of HSC activation may prevent liver fibrosis. Acetaldehyde, the most deleterious metabolite of alcohol, triggers HSC activation in alcoholic liver injury. In the present study, we investigated the protective effect of sodium ferulate (SF), a sodium salt of ferulic acid that is rich in fruits and vegetables, on acetaldehyde-stimulated HSC activation using precision-cut liver slices (PCLSs). Rat PCLSs were co-incubated with 700 mu M acetaldehyde and different concentrations of SF. Hepatotoxicity was assessed by measuring enzyme leakage and malondialdehyde content in tissue. alpha-Smooth muscle actin, transforming growth factor-beta(1), and hydroxyproline were determined to assess the activation of HSCs. In addition, matrix metalloproteinase (MMP)-1 and the tissue inhibitor of metalloproteinase (TIMP-1) were determined to evaluate collagen degradation. SF prominently prevented the enzyme leakage in acetaldehyde-treated slices and also inhibited HSC activation and collagen production stimulated by acetaldehyde. In addition, SF increased MMP-1 expression and decreased TIMP-1 expression. These results showed that SF protected PCLSs from acetaldehyde-stimulated HSC activation and liver injury, which may be associated with the attenuation of oxidative injury and acceleration of collagen degradation.