Promoter methylation and expression changes of CDH1 and P16 genes in invasive breast cancer and adjacent normal breast tissue
Promoter methylation and expression changes of CDH1 and P16 genes in invasive breast cancer and adjacent normal breast tissue
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DOI：
10.4149/neo_2010_05_465
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发表时间：
2010-01-01
期刊：
影响因子：
3
通讯作者：
Sakizli, M.
中科院分区：
文献类型：
--
作者：
Cavusoglu, A. Celebiler;Sevinc, A. I.;Sakizli, M.
我们研究了乳腺癌及其邻近正常组织与正常对照乳腺组织中 P16 和 CDH1 基因的启动子甲基化状态和表达水平，以与其组织病理学参数相关。该研究包括来自 62 名乳腺癌患者和 4 名正常对照乳腺组织的 24 个样本（肿瘤和邻近非恶性组织）。我们使用甲基化特异性 PCR 来评估甲基化状态，并使用定量 RTPCR 来测量基因表达水平。肿瘤组织中P16基因和CDH1基因甲基化发生率分别为24.2%和33.9%。 CDH1和P16基因在正常对照组织中未甲基化。 CDH1 低表达被发现与晚期、组织学类型、高肿瘤分级和淋巴结受累显着相关。发现 P16 表达与任何组织病理学参数均不显着相关。但P16过表达的病例中有60%为雌激素阴性，其中40%为组织学3级。 P16和CDH1在肿瘤组织中与癌旁正常组织以及癌旁正常组织与正常非肿瘤组织中均有不同的表达水平。
We studied the promoter methylation status and expression levels of P16 and CDH1 genes in breast cancer and their adjacent normal tissues with normal control breast tissues, to correlate with their histopathological parameters. Hundred twenty four samples (tumor and adjacent nonmalignant tissues) from 62 breast cancer patients and 4 normal control breast tissues were included in the study. We used methylation specific PCR to evaluate methylation status and quantitative RTPCR to measure the gene expression levels. Methylation incidence of P16 gene and CDH1 gene in tumor tissues were 24.2 % and 33.9 %, respectively. CDH1 and P16 gene were not methylated in normal control tissues. CDH1 underexpression is found to be significant in correlation with advanced stage, histologic type, high tumor grade and lymph node involvement. P16 expression is found not to be significantly related with any histopathological parameters. But 60% of cases which overexpresses P16 were estrogen negative, and 40% of them were histologic grade 3. Both P16 and CDH1 had different expression levels in tumor tissues compared to the adjacent normal tissues and in adjacent normal tissues compared to the normal non-tumor tissues.