Fourteen polymorphic microsatellite DNA markers for the human malaria parasite Plasmodium vivax
Fourteen polymorphic microsatellite DNA markers for the human malaria parasite Plasmodium vivax
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DOI：
10.1111/j.1471-8286.2006.01534.x
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发表时间：
2007-01-01
期刊：
影响因子：
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通讯作者：
Wirth, D. F.
中科院分区：
文献类型：
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作者：
Karunaweera, N. D.;Ferreira, M. . U.;Wirth, D. F.
我们优化了一组14个针对间日疟原虫的多态微卫星标记，所有这些标记都由三核苷酸或四核苷酸重复组成。这些标记的聚合酶链式反应扩增条件相同，用于筛选来自斯里兰卡疟疾流行区的25个寄生虫分离物。每个座位的等位基因总数为6~13个(平均7.8个)，期望杂合度为0.627~0.913(平均0.790个)。这些标记现在被用来描述其他流行地区间日疟原虫的种群结构。
We have optimized a set of 14 polymorphic microsatellite markers for the human malaria parasite Plasmodium vivax, all of them consisting of either tri- or tetranucleotide repeats. These markers, whose polymerase chain reaction amplification conditions are identical, were used to screen 25 parasite isolates from malaria-endemic areas in Sri Lanka. The total number of alleles per locus ranged between 6 and 13 (average, 7.8), and expected heterozygosity ranged from 0.627 to 0.913 (average, 0.790). These markers are now being used to characterize the population structure of P. vivax in other endemic areas.