One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device
One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device
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DOI：
10.1039/b708859k
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发表时间：
2007-01-01
影响因子：
4.9
通讯作者：
Wilson, Robert
中科院分区：
文献类型：
--
作者：
Aveyard, Jenny;Mehrabi, Maryam;Wilson, Robert
使用无抗体侧流装置检测特定的 PCR 产物，将其夹在共价连接到金纳米颗粒 (GNP) 的报告寡核苷酸之间，并捕获共价连接到硝基纤维素色谱条的寡核苷酸。
Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.