Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts.
Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts.
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DOI：
10.1038/ncomms10324
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发表时间：
2016-01-08
影响因子：
16.6
通讯作者：
Livera G
中科院分区：
文献类型：
--
作者：
Abby E;Tourpin S;Ribeiro J;Daniel K;Messiaen S;Moison D;Guerquin J;Gaillard JC;Armengaud J;Langa F;Toth A;Martini E;Livera G
有性生殖在很大程度上依赖于减数分裂，这是一种保守的、专门的细胞分裂程序，对于单倍体配子的产生至关重要。在这里，我们证明生育力和减数分裂程序的实施需要一种先前未表征的减数分裂特异性蛋白 MEIOC。小鼠减数分裂失效会导致雄性和雌性早期和多效性减数分裂缺陷。 MEIOC 可防止减数分裂转录物降解，并与结合大量减数分裂 mRNA 的 RNA 解旋酶相互作用。我们的结果表明，减数分裂的正确参与需要减数分裂转录本的特异性稳定，这是哺乳动物中以前很少被重视的特征。值得注意的是，减数分裂开始时 MEIOC 的上调不需要视黄酸和 STRA8 信号传导。因此，我们提出减数分裂程序的完全诱导需要视黄酸依赖性和非依赖性机制。鉴于 MEIOC 同源物在多细胞动物中是保守的，涉及转录后调节的后一个过程可能代表了一种祖先机制。 减数分裂是产生单倍体配子的细胞分裂程序，由视黄酸依赖性过程启动。在这里，作者报告说，减数分裂特异性蛋白 MEIOC 以不依赖视黄酸的方式上调，并且是稳定减数分裂特异性转录本所必需的。
Sexual reproduction is crucially dependent on meiosis, a conserved, specialized cell division programme that is essential for the production of haploid gametes. Here we demonstrate that fertility and the implementation of the meiotic programme require a previously uncharacterized meiosis-specific protein, MEIOC. Meioc invalidation in mice induces early and pleiotropic meiotic defects in males and females. MEIOC prevents meiotic transcript degradation and interacts with an RNA helicase that binds numerous meiotic mRNAs. Our results indicate that proper engagement into meiosis necessitates the specific stabilization of meiotic transcripts, a previously little-appreciated feature in mammals. Remarkably, the upregulation of MEIOC at the onset of meiosis does not require retinoic acid and STRA8 signalling. Thus, we propose that the complete induction of the meiotic programme requires both retinoic acid-dependent and -independent mechanisms. The latter process involving post-transcriptional regulation likely represents an ancestral mechanism, given that MEIOC homologues are conserved throughout multicellular animals. Meiosis is a cell division program that produces haploid gametes and is initiated by a retinoic acid-dependent process. Here the authors report that a meiosis-specific protein, MEIOC, is upregulated in a retinoic acid-independent manner and is required to stabilise meiosis-specific transcripts.