Neurobehavioural effects of exposure to fluoride in the earliest stages of rat development
Neurobehavioural effects of exposure to fluoride in the earliest stages of rat development
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DOI：
10.1016/j.physbeh.2015.04.044
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发表时间：
2015-08-01
影响因子：
2.9
通讯作者：
Minetti, Alejandra
中科院分区：
文献类型：
--
作者：
Bartos, Mariana;Gumilar, Fernanda;Minetti, Alejandra
众所周知，暴露于高浓度的氟化物（F）会对人类健康产生有害影响。然而，在过去的几年里，人们得出结论，低浓度的氟也可能对健康产生不利影响。已证实F可经胎盘通过并掺入胎儿组织。因此，本工作的目的是研究在妊娠期和哺乳期暴露于低水平的氟对中枢神经系统功能的影响。Wistar大鼠在怀孕和哺乳期间暴露于低氟浓度（5和10毫克/升）。对每只幼仔的感觉运动反射进行了分析，并记录了出生后眼睛和耳道打开的日期。随后分别通过旷场试验和迷宫试验分析了45天和90日龄子代的运动活动和焦虑。一个显着的延迟，在所有的后代，其母亲已暴露于两个F浓度测试的眼睛开放的发展。暴露于5和10毫克/升的氟也被发现显着减少自发活动，只有在90天大的男性和女性的后代。还检测到年轻女性和所有成年后代暴露于两种F浓度的焦虑指数较低。两者合计，本研究的结果表明，暴露于低氟浓度在怀孕和哺乳期产生中枢神经系统的机制，调节运动和敏感的发展，自发活动和焦虑功能障碍。(C)2015 Elsevier Inc. All rights reserved.
It is known that exposure to high concentrations of Fluoride (F) produces deleterious health effects in human population. However, in the last years it has been concluded that low concentrations of F may have adverse health effects as well. Transplacental passage of F and its incorporation into foetal tissues has been demonstrated. Therefore, the purpose of the present work was to study the effects of the exposure to low levels of F during pregnancy and lactation on the central nervous system functionality. Wistar rats were exposed to low F concentrations (5 and 10 mg/l) during pregnancy and lactation. Sensorimotor reflexes in the each pup were analysed and the postnatal day on which both eyes and auditory canals were opened was recorded. Locomotor activity and anxiety were subsequently analysed in 45- and 90-day-old offspring by an open field test and plus maze test, respectively. A significant delay in the development of eye opening was observed in all offspring whose mothers had been exposed to the two F concentrations tested. Exposure to 5 and 10 mg/l F was also found to significantly decrease locomotor activity only in 90-day-old male and female offspring. A low index of anxiety in the young females and in all adult offspring exposed to the two F concentrations tested was also detected. Taken together, findings from the present study show that exposure to low F concentrations during pregnancy and lactation produces dysfunction in the central nervous system mechanisms which regulate motor and sensitive development, locomotor activity and anxiety. (C) 2015 Elsevier Inc. All rights reserved.