Determination of the impact of DNA damage sites on DNA duplex stability by a competitive mobility-shift assay.
Determination of the impact of DNA damage sites on DNA duplex stability by a competitive mobility-shift assay.
复制标题
通过竞争性迁移率变化测定确定 DNA 损伤位点对 DNA 双链体稳定性的影响。
DOI：
10.1006/abio.2001.5353
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
RotiRoti,J
中科院分区：
文献类型：
--
作者：
Chen,MS;Laszlo,A;RotiRoti,J
Materials and methods. The oligonucleotides used for the analysis of the abasic site and 8-oxo-dG were synthesized based on the codon 12 (and surrounding sequence) of KRAS (Fig. 1A)(4) and on the AP-1 binding site of the NF-kappa B gene (Fig. 1B), respectively. The abasic probe was generated by digesting the uracil-containing oligonucleotide with uracil-DNA glycosylase (New England Biolabs) and then labeled with [-32P] ATP using T4 polynucleotide kinase. Probes containing 8-oxo-dG were prepared by direct incorporation
通过竞争性迁移位移测定检测单碱基突变。
影响因子：
2.9
作者：
Chen,M;Laszlo,A;Roti,JL;Hunt,CR
通讯作者：
Hunt,CR
无碱基和无核苷位点对 DNA 双链体稳定性、构象和熔解行为的影响：热力学和结构数据的相关性。
DOI：
10.1073/pnas.86.10.3614
发表时间：
1989
影响因子：
11.1
作者：
Vesnaver,G;Chang,CN;Eisenberg,M;Grollman,AP;Breslauer,KJ
通讯作者：
Breslauer,KJ