In-vivo longitudinal changes in thickness of the postnatal canine retina
In-vivo longitudinal changes in thickness of the postnatal canine retina
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DOI：
10.1016/j.exer.2020.107926
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发表时间：
2020-03-01
影响因子：
3.4
通讯作者：
Beltran, William A.
中科院分区：
文献类型：
--
作者：
Dufour, Valerie L.;Yu, Yinxi;Beltran, William A.
本工作的目的是通过光谱域光学相干断层扫描（OCT）评估外核层（ONL），ONL +感光内段（IS）的厚度变化，以及视网膜厚度，作为年龄的函数在正常犬视网膜。对17只4 ~ 119周龄正常犬的双眼进行了OCT视网膜扫描，从视神经乳头（ONH）边缘沿着上级子午线延伸。在使用Heidelberg Eye Explorer软件进行手动分割后，确定沿上级和下级区域的沿着不同参数。使用单相指数衰减模型对厚度随年龄的变化进行建模。体内OCT成像结果显示，在任何年龄，ONL、ONL + IS和视网膜厚度均无眼间统计学显著差异。然而，发现所有三个参数在上级与下级视网膜中在统计学上显著更厚。在4至12周龄之间，在上级和下层视网膜中发生三层的快速变薄，然后在约20周龄时达到平台。总之，ONL，ONL + IS，和正常犬视网膜的视网膜厚度在出生后的前三个月内显著降低，这可能是由于眼体积和感光细胞的切线分散的整体增加，因为早期感光细胞发育细胞死亡在该年龄是非常有限的。ONL、ONL + IS和视网膜变薄的自然史的建立将允许更准确地评估视网膜退行性疾病的进展，并有助于检测在该大型动物模型中评价的新型视网膜治疗的积极挽救作用。
The objectives of the present work were to assess by spectral domain optical coherence tomography (OCT) the changes in thickness of the outer nuclear layer (ONL), the ONL + photoreceptor inner segment (IS), and the retinal thickness, as a function of age in the normal canine retina. OCT retinal scans extending from the edge of the optic nerve head (ONH) along the superior and inferior meridians were captured in both eyes of 17 normal dogs at age ranging from 4 to 119 weeks. The different parameters along the superior and the inferior regions were determined following manual segmentation using the Heidelberg Eye Explorer software. Changes in thickness with age were modeled using one-phase exponential decay models. In vivo OCT imaging results showed no interocular statistically significant differences in ONL, ONL + IS, and retinal thickness at any age. All three parameters were however found to be statistically significantly thicker in the superior vs inferior retina. A rapid thinning of the three layers occurs in both the superior and inferior retina between 4 and 12 weeks of age, before reaching a plateau at around 20 weeks of age. In conclusion, the ONL, ONL + IS, and retinal thickness of the normal canine retina decrease significantly during the first three postnatal months, and is likely attributed to an overall increase in the eye volume and tangential dispersion of the photoreceptor since early photoreceptor developmental cell death is very limited at that age. Establishment of the natural history of ONL, ONL + IS, and retinal thinning will allow a more accurate assessment of the progression of a retinal degenerative condition as well as facilitate the detection of positive rescue effect of novel retinal therapies evaluated in this large animal model.