Outer dense fibre proteins from human sperm tail:: molecular cloning and expression analyses of two cDNA transcripts encoding proteins of ∼70 kDa
Outer dense fibre proteins from human sperm tail:: molecular cloning and expression analyses of two cDNA transcripts encoding proteins of ∼70 kDa
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DOI：
10.1093/molehr/5.7.627
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发表时间：
1999-07-01
影响因子：
4
通讯作者：
Hoyer-Fender, S
中科院分区：
文献类型：
--
作者：
Peterson, C;Füzesi, L;Hoyer-Fender, S
外致密纤维（ODF）是精子尾部的主要细胞骨架结构。尽管它们在精子尾部的形态和功能中很重要，但它们的成分描述得很少。在这里，我们调查的蛋白质组成的人类外层致密纤维。我们的研究结果表明，人ODF由约10个主要和至少15个次要蛋白质组成，其中所有主要蛋白质都是ODF 1，ODF 2或ODF 2相关蛋白。从人睾丸cDNA文库中，我们分离到两个略有不同的cDNA编码的ODF 2蛋白的相似的70 kDa。人ODF 2 cDNA及其编码的蛋白质与从大鼠和小鼠中分离的那些非常相似，这表明高进化压力！存在于这些蛋白质中。ODF 2的转录仅限于睾丸组织，更具体地说，如通过非放射性原位杂交所证明的那样，仅限于圆形精子细胞。精子尾部检测到ODF 2蛋白。它们沿着精子尾部长度的分布表明，ODF通常延伸到精子尾部主段的一半左右。前一个结果打开了可能性的筛选有关的精子尾部蛋白的分布与运动障碍。
The outer dense fibres (ODF) are a main cytoskeletal structure of the sperm tail. Despite their importance in the morphology and function of the sperm tail, their constituents are poorly described. Here we investigate the protein composition of human outer dense fibres. Our results suggest that human ODF consist of about 10 major and of at least 15 minor proteins, where all major proteins are ODF1, ODF2 or ODF2-related proteins. From a human testis cDNA library, we isolated two slightly different cDNAs encoding ODF2 proteins of similar to 70 kDa. Human ODF2 cDNAs and their encoded proteins are very similar to those isolated from rat and mouse pointing to a high evolutionary pressure! residing on these proteins. Transcription of ODF2 is restricted to testis tissue and more specifically to round spermatids as was demonstrated by a non-radioactive in-situ hybridization. ODF2 proteins were detected in the sperm tail. Their distribution along the length of the sperm tail shows that the ODF normally extend to about half the principal piece of the sperm tail. The former result opens the possibility for a screening regarding the distribution of sperm tail proteins related to motility disorders.