Age-related glycine receptor subunit changes in the cochlear nucleus of Fischer-344 rats.
Age-related glycine receptor subunit changes in the cochlear nucleus of Fischer-344 rats.
复制标题
Fischer-344 大鼠耳蜗核中与年龄相关的甘氨酸受体亚基变化。
DOI：
10.1097/00005537-199801000-00005
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Helfert,RH
中科院分区：
文献类型：
--
作者：
Krenning,J;Hughes,LF;Caspary,DM;Helfert,RH
先前的研究表明，Fischer（F344）大鼠耳蜗核（CN）中士的宁敏感性甘氨酸受体的结合水平随年龄增长而降低。鉴于甘氨酸在正常CN功能中发挥的主要作用，甘氨酸受体活性的变化可能有助于中枢性老年性耳聋。为了进一步评估年龄对甘氨酸受体的影响，原位杂交被用来评估，在三个年龄组的F344大鼠，其四个亚基的基因表达水平的变化。与3月龄大鼠相比，18月龄和27月龄大鼠前腹侧CN中α 1和β亚基的mRNA表达显著降低，而α 2亚基的mRNA表达增加。如果蛋白质表达相似，这些亚基的变化可能会改变甘氨酸受体的功能，从而影响与其配体的结合。
Previous studies have shown that levels of binding for the strychnine‐sensitive glycine receptor in the cochlear nucleus (CN) of Fischer(F344) rats decrease with age. Given the major role glycine plays in normal CN function, changes in glycine‐receptor activity may contribute to central presbycusis. To further evaluate the impact of age on glycine receptors, in situ hybridization was used to assess, in three age groups of F344 rats, changes in levels of gene expression for four of its subunits. When compared with the 3‐month‐old rats, expression of mRNAs for α1and β subunits in the anteroventral CN decreased significantly in the 18‐ and 27‐month‐old age groups, while mRNA expression for the α2subunit increased. If protein expressions are similar, these subunit changes may alter the function of glycine receptors, thereby affecting binding to its ligands.