A quartz nanopillar hemocytometer for high-yield separation and counting of CD4+ T lymphocytes
A quartz nanopillar hemocytometer for high-yield separation and counting of CD4+ T lymphocytes
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DOI：
10.1039/c2nr11338d
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发表时间：
2012-01-01
期刊：
影响因子：
6.7
通讯作者：
Lee, Sang-Kwon
中科院分区：
文献类型：
--
作者：
Kim, Dong-Joo;Seol, Jin-Kyeong;Lee, Sang-Kwon
We report the development of a novel quartz nanopillar (QNP) array cell separation system capable of selectively capturing and isolating a single cell population including primary CD4(+) T lymphocytes from the whole pool of splenocytes. Integrated with a photolithographically patterned hemocytometer structure, the streptavidin (STR)-functionalized-QNP (STR-QNP) arrays allow for direct quantitation of captured cells using high content imaging. This technology exhibits an excellent separation yield (efficiency) of similar to 95.3 +/- 1.1% for the CD4(+) T lymphocytes from the mouse splenocyte suspensions and good linear response for quantitating captured CD4(+) T-lymphoblasts, which is comparable to flow cytometry and outperforms any non-nanostructured surface capture techniques, i.e. cell panning. This nanopillar hemocytometer represents a simple, yet efficient cell capture and counting technology and may find immediate applications for diagnosis and immune monitoring in the point-of-care setting.
