Effect of diet on insulin binding and glucose transport in rat sarcolemmal vesicles.
Effect of diet on insulin binding and glucose transport in rat sarcolemmal vesicles.
复制标题
饮食对大鼠肌膜囊泡中胰岛素结合和葡萄糖转运的影响。
DOI：
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发表时间：
1987
影响因子：
--
通讯作者：
S. Kaplan
中科院分区：
文献类型：
--
作者：
G. Grimditch;R. Barnard;E. Sternlicht;R. Whitson;S. Kaplan
本研究的目的是比较高脂肪，高蔗糖饮食（HFS）和低脂，高复合碳水化合物饮食（LFC）对大鼠骨骼肌葡萄糖耐量，胰岛素结合和葡萄糖转运的影响。在静脉葡萄糖耐量试验中，HFS组5分钟时的峰值葡萄糖值显著较高; 0分钟、20分钟和60分钟的值相似。HFS组在所有时间点（60分钟除外）的胰岛素值均显著较高，表明全身胰岛素抵抗。骨骼肌是造成这种胰岛素抵抗的部分原因，因为在基础条件下，LFC和HFS大鼠之间分离的肌膜（SL）囊泡中的特异性D-葡萄糖转运相似（35 +/- 5 vs. 32 +/- 4 pmol/mg蛋白），尽管血浆胰岛素水平较高。对胰岛素与肌膜囊泡结合曲线的Scatchard分析显示，HFS饮食显著降低了高亲和力结合位点的Ka（0.63 +/- 0.09 vs. 0.35 +/- 0.05 X 10（9）M-1）;未观察到其他结合变化。在HFS组中，在最大胰岛素刺激（1 U/kg）后SL囊泡中的特异性D-葡萄糖转运显著抑制（87 +/- 7 pmol/mg蛋白对58 +/- 7 pmol/mg蛋白），表明HFS喂养也引起了结合后缺陷。这些结果表明，与HFS饮食相关的骨骼肌胰岛素抵抗是由于高亲和力胰岛素受体的Ka降低和后结合缺陷。
The purpose of this study was to compare the effects of a high-fat, high-sucrose diet (HFS) and a low-fat, high-complex carbohydrate diet (LFC) on glucose tolerance, insulin binding, and glucose transport in rat skeletal muscle. During the intravenous glucose tolerance test, peak glucose values at 5 min were significantly higher in the HFS group; 0-, 20-, and 60-min values were similar. Insulin values were significantly higher in the HFS group at all time points (except 60 min), indicating whole-body insulin resistance. Skeletal muscle was responsible, in part, for this insulin resistance, because specific D-glucose transport in isolated sarcolemmal (SL) vesicles under basal conditions was similar between LFC and HFS rats (35 +/- 5 vs. 32 +/- 4 pmol/mg protein), despite the higher plasma insulin levels. Scatchard analyses of insulin binding curves to sarcolemmal vesicles revealed that the Ka of the high-affinity binding sites was significantly reduced by the HFS diet (0.63 +/- 0.09 vs. 0.35 +/- 0.05 X 10(9) M-1); no other binding changes were noted. Specific D-glucose transport in SL vesicles after maximum insulin stimulation (1 U/kg) was significantly depressed in the HFS group (87 +/- 7 vs. 58 +/- 7 pmol/mg protein), indicating that HFS feeding also caused a postbinding defect. These results indicate that the insulin resistance in skeletal muscle associated with a HFS diet is due to both a decrease in the Ka of the high-affinity insulin receptors and a postbinding defect.
膳食碳水化合物对骨骼肌葡萄糖摄取的影响。
DOI：
10.1093/ajcn/41.3.526
发表时间：
1985
期刊：
The American journal of clinical nutrition
影响因子：
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作者：
Miller,WJ;Sherman,WM;Dodd,H;Ivy,JL
通讯作者：
Ivy,JL