ORGANIZATION OF THE NUCLEAR-PORE COMPLEX IN SACCHAROMYCES-CEREVISIAE
ORGANIZATION OF THE NUCLEAR-PORE COMPLEX IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0889-1605(89)90047-5
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发表时间：
1989-08-01
期刊：
影响因子：
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通讯作者：
DOUGLAS, MG
中科院分区：
文献类型：
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作者：
ALLEN, JL;DOUGLAS, MG
已经从酿酒酵母中分离出富含核孔复合物（NPC）的级分。用去污剂、核酸酶和盐连续提取细胞核揭示了与其他真核生物相似的酵母NPC组织。酵母NPC含有30 nm的“环”结构，以前在其他生物中没有描述过。这种结构似乎将10 nm的细丝组织成具有八重旋转对称性的组装体。NPC组分中的一些蛋白质能够形成中等大小的细丝。这些研究表明，核孔复合物的某些成分组织了核和细胞质中间丝网络之间的相互作用。
Fractions enriched for nuclear pore complexes (NPCs) have been isolated from Saccharomyces cerevisiae. The sequential extraction of nuclei with detergent, nucleases, and salt reveals an organization of the yeast NPC similar to other eukaryotes. Yeast NPCs contain a 30-nm "ring" structure not previously described in other organisms. This structure appears to organize 10-nm filaments into an assembly which exhibits an eight-fold rotational symmetry. Some proteins in the NPC fraction are capable of forming intermediate-sized filaments. These studies suggest that some component of the nuclear pore complex organizes an interaction between nuclear and cytoplasmic networks of intermediate filaments.