Comparison of Two Cellulomonas Strains and Their Interaction with Azospirillum brasilense in Degradation of Wheat Straw and Associated Nitrogen Fixation
Comparison of Two Cellulomonas Strains and Their Interaction with Azospirillum brasilense in Degradation of Wheat Straw and Associated Nitrogen Fixation
复制标题
两种纤维单胞菌菌株的比较及其与巴西固氮螺菌在麦秆降解和相关固氮方面的相互作用
DOI：
10.1128/aem.51.4.855-861.1986
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发表时间：
1986
影响因子：
4.4
通讯作者：
A. H. Gibson
中科院分区：
文献类型：
--
作者：
D. M. Halsall;A. H. Gibson
将纤维素单胞菌sp. CS1-17突变株与纤维素单胞菌gelida 2480作为混合培养的纤维素分解组分进行比较，该混合培养负责分解麦秸以支持巴西偶氮螺旋菌Sp7 （ATCC 29145）的非共生固氮。在低氧条件下，胞单胞菌（CSI-17）的纤维素分解效率高于胞单胞菌（C. gelida），在与巴西单胞菌（A. brasilense）混合培养时，胞单胞菌（C2H2还原）的固氮活性更高，且以秸秆为碳源的固氮能力更强。秸秆分解的重量测定和全氮测定结果表明，含纤维素单胞菌和格里达菌的混合物的固氮效率分别为72和63 mg / g。纤维素单胞菌和偶氮螺旋菌均表现出广泛的pH耐受性。将纤维素单胞菌-巴西氮螺旋菌组合引入无菌土壤中，在pH为7.0时比在原生土壤pH（5.6）时更有效地固氮。这种土壤的本地重氮营养微生物群也是如此。讨论了这项工作对现场情况的潜在影响。
A mutant strain of Cellulomonas sp. CS1-17 was compared with Cellulomonas gelida 2480 as the cellulolytic component of a mixed culture which was responsible for the breakdown of wheat straw to support asymbiotic nitrogen fixation by Azospirillum brasilense Sp7 (ATCC 29145). Cellulomonas sp. strain CSI-17 was more efficient than was C. gelida in cellulose breakdown at lower oxygen concentrations and, in mixed culture with A. brasilense, it supported higher nitrogenase activity (C2H2 reduction) and nitrogen fixation with straw as the carbon source. Based on gravimetric determinations of straw breakdown and total N determinations, the efficiency of nitrogen fixation was 72 and 63 mg of N per g of straw utilized for the mixtures containing Cellulomonas sp. and C. gelida, respectively. Both Cellulomonas spp. and Azospirillum spp. exhibited a wide range of pH tolerance. When introduced into sterilized soil, the Cellulomonas sp.-Azospirillum brasilense association was more effective in nitrogen fixation at a pH of 7.0 than at the native soil pH (5.6). This was also true of the indigenous diazotrophic microflora of this soil. The potential implications of this work to the field situation are discussed.