Distinct responses of Purkinje neurons and roles of simple spikes during associative motor learning in larval zebrafish
Distinct responses of Purkinje neurons and roles of simple spikes during associative motor learning in larval zebrafish
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DOI：
10.7554/elife.22537
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发表时间：
2017-05-25
期刊：
影响因子：
7.7
通讯作者：
Raman, Indira M.
中科院分区：
文献类型：
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作者：
Harmon, Thomas C.;Magaram, Uri;Raman, Indira M.
为了研究学习过程中小脑的活动，我们从幼斑马鱼浦肯野细胞进行全细胞记录，同时监测联想条件反射过程中的虚构游泳。鱼学习游泳的视觉刺激反应之前触觉刺激的尾巴。小脑消融术消除了学习能力。所有浦肯野细胞都表现出与任务相关的活动。根据学习游泳过程中出现的复杂锋电位的多少，将其分为多个、单个和零个复杂锋电位（MCS、SCS、ZCS）细胞。随着学习，MCS和ZCS细胞在视觉刺激过程中增加了攀爬纤维（MCS）或平行纤维（ZCS）的输入; SCS细胞发射了与学习游泳事件相关的复杂尖峰。类别与位置相关。光遗传学抑制简单的尖峰，只有在视觉刺激表明，简单的尖峰需要收购和学习反应的表达的早期阶段，但不是他们的维护，与短暂的，指导性的作用，简单的尖峰在幼虫斑马鱼小脑学习。
To study cerebellar activity during learning, we made whole-cell recordings from larval zebrafish Purkinje cells while monitoring fictive swimming during associative conditioning. Fish learned to swim in response to visual stimulation preceding tactile stimulation of the tail. Learning was abolished by cerebellar ablation. All Purkinje cells showed task-related activity. Based on how many complex spikes emerged during learned swimming, they were classified as multiple, single, or zero complex spike (MCS, SCS, ZCS) cells. With learning, MCS and ZCS cells developed increased climbing fiber (MCS) or parallel fiber (ZCS) input during visual stimulation; SCS cells fired complex spikes associated with learned swimming episodes. The categories correlated with location. Optogenetically suppressing simple spikes only during visual stimulation demonstrated that simple spikes are required for acquisition and early stages of expression of learned responses, but not their maintenance, consistent with a transient, instructive role for simple spikes during cerebellar learning in larval zebrafish.