Quantitative assessment of directed hind limb scratching behavior as a rodent itch model
Quantitative assessment of directed hind limb scratching behavior as a rodent itch model
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DOI：
10.1016/s0165-0270(03)00074-8
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发表时间：
2003-06-30
影响因子：
3
通讯作者：
Carstens, E
中科院分区：
文献类型：
--
作者：
Nojima, H;Carstens, E
在啮齿类动物中，后肢抓挠越来越多地被用作瘙痒模型。划痕通常被量化为60分钟内的划痕次数。由于抓挠的止痒效果应取决于皮肤接触的总时间，那么抓挠的持续时间和抓挠次数也可能是重要的因素。因此，我们在SD大鼠皮内注射5-羟色胺的部位(颈背)的抓挠过程中测量了这些参数。5-羟色胺引起的抓挠次数明显多于生理盐水。随着时间的推移，有一个两相的抓挠模式，峰值出现在10-20分钟和40-50分钟。虽然累计比赛持续时间(2分钟间隔)具有类似的双相分布，但平均个人比赛持续时间(2.1%S)并没有随着时间的推移而显著变化。在8赫兹时，圈内抓挠频率随着时间的推移保持不变。纳曲酮(3 mg/kg和5 mg/kg)可剂量依赖性地抑制小鼠的抓挠次数，但单次抓挠持续时间和抓挠次数与未加纳曲酮的5-羟色胺诱导的抓挠次数无明显差异。这些结果验证了抓挠次数作为瘙痒相关抓挠程度的指标的有效性。(C)2003 Elsevier Science B.V.保留所有权利。
Hind limb scratching is used increasingly as an itch model in rodents. Scratching is usually quantified as the number of scratching bouts over a 60 min period. Since the antipruritic effect of scratching should depend on the total time of skin contact, then the duration of scratching bouts and within-bout scratching frequency may also be important factors. Therefore, we measured these parameters during episodes of scratching directed toward the site (nape of neck) of intradermal injection of serotonin in Sprague-Dawley rats. Serotonin elicited significantly more scratching bouts than saline. There was a biphasic pattern of scratching over time, with peaks at 10-20 and 40-50 min. Although cumulative bout duration (2-min intervals) had a similar biphasic distribution, the mean individual bout duration (2.1 s) did not change significantly over time. Within-bout scratching frequency remained constant over time at 8 Hz. The number of scratching bouts was suppressed in a dose dependent manner by naltrexone (3 and 5 mg/kg), while the individual bout duration and the within-bout frequency were not significantly different compared with serotonin-evoked scratching without naltrexone. These results Validate the total number of scratching bouts as an indicator of the magnitude of itch-related scratching. (C) 2003 Elsevier Science B.V. All rights reserved.