LncRNA PRNCR1 promoted the progression of eclampsia by regulating the MAPK signal pathway
LncRNA PRNCR1 promoted the progression of eclampsia by regulating the MAPK signal pathway
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DOI：
10.26355/eurrev_201806_15240
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发表时间：
2018-06-01
影响因子：
3.3
通讯作者：
Huang, Y.
中科院分区：
文献类型：
--
作者：
Jiao, S.;Wang, S. -Y.;Huang, Y.
目的：探讨lncRNA PRNCR1在子痫前期（PE）中的表达水平和生物学功能。 患者和方法：本研究纳入57名PE患者和57名正常孕妇。采用qRT-PCR检测PE患者及正常妊娠孕妇胎盘中PRNCR1的表达水平。进行CCK-8测定以确定滋养层上PRNCR1干扰和过表达后的细胞活力。采用Western blot检测PRNCR1蛋白水平。结果：PE组收缩压、舒张压和尿蛋白水平均高于正常孕妇，而PE组新生儿体重明显低于正常孕妇。 LncRNA PRNCR1在PE患者中过度表达，与PE患者的收缩压、舒张压和尿蛋白呈正相关，而与胎儿出生体重呈负相关。此外，干扰PRNCR1后，BeWo滋养层细胞中PRNCR1的表达显着降低，而细胞活力增加。然而，HTR-8 细胞中 PRNCR1 的过度表达显着降低了细胞的活力。 PE患者中p-p38、p-JNK的表达水平高于正常孕妇，p-ERK的表达水平降低，提示PRNCR1通过调节MAPK信号通路促进PE的进展。结论：PRNCR1在PE中高表达，并通过调节MAPK信号通路促进PE的进展。
OBJECTIVE: We aimed to explore the expression level and biological function of lncRNA PRNCR1 in preeclampsia (PE).PATIENTS AND METHODS: 57 PE patients and 57 normal pregnant women were enrolled in this study. The expression level of PRNCR1 in the maternal placenta of PE patients and normal pregnancy was detected by qRT-PCR. The CCK-8 assay was carried out to determine the cell viability after interference and overexpression of PRNCR1 on trophoblasts. We utilized Western blot to examine the protein level of PRNCR1.RESULTS: Higher systolic blood pressure, diastolic blood pressure and urinary protein levels in PE patients were observed in comparison with those in normal pregnant women, while the neonatal weight in PE group was markedly lower than that in normal pregnant women. LncRNA PRNCR1 was overexpressed in PE patients, which was positively correlated with systolic blood pressure, diastolic blood pressure and urine protein, whereas negatively correlated with fetal birth weight of PE patients. In addition, the expression of PRNCR1 in BeWo trophoblast cells was significantly decreased after the interference with PRNCR1, while the cell viability increased. However, overexpression of PRNCR1 in HTR-8 cells significantly reduced the viability of cells. Expression levels of p-p38 and p-JNK in PE patients were higher than those in normal pregnancy women, and the expression level of p-ERK was decreased, which suggested that PRNCR1 promoted the progression of PE by modulating the MAPK signaling pathway.CONCLUSIONS: PRNCR1 is highly expressed in PE and promotes the progression of PE by modulating the MAPK signaling pathway.