Rhizobium rhizophilum sp. nov., an indole acetic acid-producing bacterium isolated from rape (Brassica napus L.) rhizosphere soil
Rhizobium rhizophilum sp. nov., an indole acetic acid-producing bacterium isolated from rape (Brassica napus L.) rhizosphere soil
复制标题
根瘤菌 sp.
DOI：
10.1099/ijsem.0.004374
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发表时间：
2020-01-01
影响因子：
2.8
通讯作者：
Sun, Jian-guang
中科院分区：
文献类型：
--
作者：
Gao, Jun-lian;Wang, Li-wei;Sun, Jian-guang
从Yakeshi市油菜根际分离到一株革兰氏阴性、需氧棒状吲哚乙酸产菌，命名为7209-2(T)。内蒙古，中国。165 rRNA基因序列分析表明，菌株7209-2(T)属于根瘤菌属，与蔷薇根瘤菌W3(T)、ipomoeae shin9-1(T)和芜州根瘤菌W44(T)亲缘关系较近，序列相似性分别为98.2%、98.1和97.9%。基于recA和atpD基因序列的系统发育分析表明，菌株7209-2(T)与wuzhourr . W44(T)和rosettiformans . W3(T)同属一个类群，序列相似性分别为92.6%和91.1%。菌株7209-2(T)的基因组大小为5.25 Mb，包含5027个预测基因，DNA G+C含量为61.2mol%。7209-2(T)与参考菌株亲缘关系最密切的物种的平均核苷酸同源性和数字DNA-DNA杂交比较值均低于物种区分的可接受阈值。菌株7209-2(T)的主要脂肪酸为特征8 （C-18:1 ω 7c和/或C-18:1 ω 6c）和特征2（C-12:(0)醛和/或未知10.953）。主要极性脂质由磷脂酰乙醇胺、二磷脂酰甘油组成。磷脂酰甘油，磷脂酰胆碱和一种未识别的氨基磷脂。主要的泛醌为醌10。综合以上结果，菌株7209-2(T)为根瘤菌属新种，建议以菌株7209-2(T) (=CGMCC 1.15691（T)=DSM 103161(T)）为类型菌株命名为Rhizobium rhizophilum sp. nov。
A novel Gram-stain-negative, aerobic, rod-shaped and indole acetic acid-producing strain, designated 7209-2(T), was isolated from rhizosphere of rape (Brassica napus L.) grown in the Yakeshi City. Inner Mongolia, PR China. The 165 rRNA gene sequence analysis indicated that strain 7209-2(T) belongs to the genus Rhizobium and is closely related to Rhizobium rosettiformans W3(T), Rhizobium ipomoeae shin9-1(T) and Rhizobium wuzhouense W44(T) with sequence similarities of 98.2, 98.1 and 97.9%, respectively. Phylogenetic analysis based on concatenated housekeeping recA and atpD gene sequences showed that strain 7209-2(T) formed a group together with R. wuzhouense W44(T) and R. rosettiformans W3(T), with sequences similarities of 92.6 and 91.1%, respectively. The genome size of strain 7209-2(T) was 5.25 Mb, comprising 5027 predicted genes with a DNA G+C content of 61.2mol%. The average nucleotide identity and digital DNA-DNA hybridization comparisons among 7209-2(T) and reference strains for the most closely related species showed values below the accepted threshold for species discrimination. The major fatty acids of strain 7209-2(T) were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c) and summed feature 2 (C-12:(0) aldehyde and/or unknown 10.953) . The major polar lipids were found to consist of phosphatidylethanolamine, diphosphatidylglycerol. phosphatidylglycerol, phosphatidylcholine and an unidentified aminophospholipid. The predominant ubiquinone was identified as quinone 10. Based on all the above results, strain 7209-2(T) represents a novel species of the genus Rhizobium, for which the name Rhizobium rhizophilum sp. nov. is proposed with 7209-2(T) (=CGMCC 1.15691(T)=DSM 103161(T)) as the type strain.