Error-free and error-prone DNA repair gene expression data through reprogramming and passage in human iPS cells
Error-free and error-prone DNA repair gene expression data through reprogramming and passage in human iPS cells
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通过在人类 iPS 细胞中重编程和传代，实现无错和易错 DNA 修复基因表达数据
DOI：
10.1016/j.dib.2020.105228
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发表时间：
2020
期刊：
影响因子：
1.2
通讯作者：
Okuzaki D
中科院分区：
文献类型：
--
作者：
Yoshimura Y;Okuzaki D
我们最近发现，DNA修复相关的基因表达可以通过重编程以及增加准确表达基因组信息的基因的表达来改变，例如同源重组（HR）和错配修复（MMR），以及减少易错跨损伤合成（TLS）聚合酶的表达。在这里，我们在另一个细胞系中证实了这种表达变化，并发现这种变化通过重叠传代以及OCT 3/4和NANOG来维持。我们的研究结果表明，与重编程相关的DNA修复相关基因表达的变化及其维持可以成为表现出多能性的细胞的质量控制的新指标。
We recently found that DNA repair-related gene expression could be altered by reprogramming as well as the increased expression of genes that accurately convey genomic information, such as homologous recombination (HR) and mismatch repair (MMR), and the decreased expression of error-prone translesion synthesis (TLS) polymerase. Here, we confirmed this change in expression in another cell-line and found that such alteration was maintained by overlapping passages as well as OCT3/4 and NANOG. Our findings suggest that changes in the expression of DNA repair-related genes associated with reprogramming and their maintenance can be novel indicators of the quality control of the cells exhibiting pluripotency.