Serum IgG antibody to the outer membrane proteins of Campylobacter pylori in children with gastroduodenal disease.
Serum IgG antibody to the outer membrane proteins of Campylobacter pylori in children with gastroduodenal disease.
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胃十二指肠疾病儿童幽门弯曲杆菌外膜蛋白血清 IgG 抗体。
DOI：
10.1093/infdis/159.3.586
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Aronoff,S
中科院分区：
文献类型：
--
作者：
Czinn,S;Carr,H;Sheffler,L;Aronoff,S
材料和方法从消化内科保存的血清中回收在彩虹婴幼儿医院接受上消化道内窥镜检查以评估胃十二指肠疾病(恶心、呕吐、呕血和上腹部腹痛)的5-18岁儿童的血清。所有样本均在内窥镜检查中获得，并保存在-70℃的100度等份中。所有患者的组织样本均检测尿素酶产生，培养幽门螺杆菌，并用上述方法镜检弯曲杆菌[3]。根据菌落形态鉴定内窥镜检查中发现的细菌为幽门螺杆菌。革兰氏染色，以及尿素酶、过氧化氢酶和氧化酶的产生[4，9]。用于定量和定性抗体检测的幽门螺杆菌试验菌株是一株临床分离自1例胃炎患儿的胃活检标本。整个细胞都能发出声响。将试验菌株接种到哥伦比亚琼脂(底特律Difco)上，含5 0只绵羊血液，在37℃微需氧培养96h。在PBS中收集微生物，所得悬浮液在4℃超声处理，通过离心法清除细胞碎片。这些全细胞超声波储存在100^il等份中，温度为-70℃，外膜制剂。细胞包膜是
Materials and MethodsSera from children 5-18 y old who underwent upper en-doscopy at Rainbow Babies and Childrens Hospital for evaluation of gastroduodenal disease (nausea, vomiting, hematemesis, and epigastric abdominal pain) were recov-ered from the collection of banked sera maintained by the Division of Gastroenterology. All samples were obtained at endoscopy and stored in 100^ iL aliquots at-70 C. Tissue specimens from all patients were tested for urease production, cultured for C. pylori, and examined microscopically for Campylobacter by methods previously described [3].Bacteria recovered at endoscopy were identified as C. pylori based on colony morphology. Gram's stain, and the production of urease, catalase, and oxidase [4, 9]. the test strain of C. pylori used for the quantitative and qualitative antibody assays was a clinical isolate from the gastric biopsy of a pediatric patient with gastritis. Whole cell sonicates. The test strain was inoculated onto Columbia agar (Difco, Detroit) containing 5^ 0 sheep's blood and incubated microaerophillically at 37 C for 96 h. The organisms were harvested in PBS and the resulting suspensions were sonicated at 4 C and cleared of cellular debris by centrifugation. These whole-cell sonicates were stored in 100^ iL aliquots at-70 C. Outer membrane preparations. Cell envelopes were