Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli
Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli
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DOI：
10.1073/pnas.0701579104
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发表时间：
2007-04-10
影响因子：
11.1
通讯作者：
Silhavy, Thomas J.
中科院分区：
文献类型：
--
作者：
Sklar, Joseph G.;Wu, Tao;Silhavy, Thomas J.
革兰氏阴性菌的外膜（OM）的主要作用是为细胞提供保护性渗透屏障，并且OM的适当维持是细胞活力所需的。OM生物发生需要通过专用的OM组装机制协调组装组成脂质和蛋白质。我们先前已经表明，在大肠杆菌中，多组分YaeT复合物负责OM β-桶蛋白（OMP）的组装。该复合物含有OMP YaeT和三种OM脂蛋白。在这里，我们报告的另一个组成部分的YaeT复合物，OM脂蛋白小蛋白A（SmpA）。在smpA中携带功能丧失突变的菌株是可行的，但在OMP组装中表现出缺陷。生化实验表明，SmpA参与维持复合物的稳定性。总之，这些实验建立了一个重要的作用，SmPA的结构和功能的YaeT复合物。
A major role of the outer membrane (OM) of Gram-negative bacteria is to provide a protective permeability barrier for the cell, and proper maintenance of the OM is required for cellular viability. OM biogenesis requires the coordinated assembly of constituent lipids and proteins via dedicated OM assembly machineries. We have previously shown that, in Escherichia coli, the multicomponent YaeT complex is responsible for the assembly of OM beta-barrel proteins (OMPs). This complex contains the OMP YaeT and three OM lipoproteins. Here, we report another component of the YaeT complex, the OM lipoprotein small protein A (SmpA). Strains carrying loss-of-function mutations in smpA are viable but exhibit defects in OMP assembly. Biochemical experiments show that SmpA is involved in maintaining complex stability. Taken together, these experiments establish an important role for SmpA in both the structure and function of the YaeT complex.