Peripheral Lymphocyte Subsets as a Marker of Parkinson's Disease in a Chinese Population
Peripheral Lymphocyte Subsets as a Marker of Parkinson's Disease in a Chinese Population
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外周淋巴细胞亚群作为中国人群帕金森病的标志物
DOI：
10.1007/s12264-017-0163-9
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发表时间：
2017-10-01
影响因子：
5.6
通讯作者：
Xu, Pingyi
中科院分区：
文献类型：
--
作者：
Cen, Luan;Yang, Chaohao;Xu, Pingyi
在这项研究中，我们对268例帕金森病（PD）患者的淋巴细胞亚型进行了临床分析，以评估其作为中国晚期帕金森病潜在标志物的临床影响。参与者包括268名散发性PD患者和268名健康对照者。采用免疫染色和流式细胞术检测外周血自然杀伤细胞（NK）和CD3+、CD3+CD4+、CD3+CD8+、CD19+淋巴细胞的数量，并计算CD+ T细胞的百分比。采用流式细胞术检测64例PD患者和46例对照者的调节性T (Treg)/辅助性T 17 （Th17）淋巴细胞的比例。结果显示，晚期PD患者NK细胞比例高于对照组（22.92% +/- 10.08% vs 19.76% +/- 10.09%, P = 0.006）， CD3+ T细胞比例降低（62.93% +/- 9.27% vs 65.75% +/- 9.13%, P = 0.005）。男性患者的CD19+ B细胞比例低于女性患者（P = 0.021），而NK细胞比例高于女性患者（P < 0.0001）。晚发性PD患者UPDRS评分和非运动症状评分均显著高于早发性PD患者（P = 0.024和P = 0.007）。UPDRS评分bbbb24的患者中CD19+ B细胞的百分比低于评分< 24的患者（10.17% +/- 4.19% vs 12.22% +/- 5.39%, P = 0.009）。此外，女性患者Treg/Th17比值高于女性对照组（13.88 +/- 6.32 vs 9.94 +/- 4.06, P = 0.042）。这些结果表明，外周血NK细胞、CD3+ T细胞和CD19+ B细胞的百分比以及Treg/Th17比值可用于预测中国人帕金森病的风险，并为新的诊断生物标志物和治疗设计提供新的途径。
In this study, we conducted a clinical analysis of lymphocyte subtypes in 268 patients with Parkinson's disease (PD) to assess their clinical impact as a potential marker of advanced PD in Chinese patients. The participants comprised 268 sporadic PD patients and 268 healthy controls. The numbers of natural killer (NK) cells and CD3+, CD3+CD4+, CD3+CD8+, and CD19+ lymphocytes from peripheral blood were determined by immunostaining and flow cytometric analysis and the percentages of these CD+ T cells were calculated. The ratio of regulatory T (Treg)/helper T 17 (Th17) lymphocytes from 64 PD patients and 46 controls was determined by flow cytometric analysis. The results showed that the percentage of NK cells was higher in advanced PD patients than in controls (22.92% +/- 10.08% versus 19.76% +/- 10.09%, P = 0.006), while CD3+ T cells are decreased (62.93% +/- 9.27% versus 65.75% +/- 9.13%, P = 0.005). The percentage of CD19+ B cells in male patients was lower (P = 0.021) than in female patients, whereas NK cells were increased (P < 0.0001). The scores on the Unified Parkinson's Disease Rating Scale (UPDRS) and the Non-Motor Symptoms Scale in late-onset PD patients were significantly higher than those in early-onset patients (P = 0.024 and P = 0.007, respectively). The percentage of CD19+ B cells in patients with UPDRS scores > 24 was lower than in those with scores < 24 (10.17% +/- 4.19% versus 12.22% +/- 5.39%, P = 0.009). In addition, the Treg/Th17 ratio in female patients was higher than that in female controls (13.88 +/- 6.32 versus 9.94 +/- 4.06, P = 0.042). These results suggest that the percentages of NK cells, CD3+ T cells, and CD19+ B cells along with the Treg/Th17 ratio in peripheral blood may be used to predict the risk of PD in Chinese individuals and provide fresh avenues for novel diagnostic biomarkers and therapeutic designs.