PGFM (13,14-dihydro-15-keto-PGF2α) in pregnant and pseudo-pregnant Iberian lynx: A new noninvasive pregnancy marker for felid species
PGFM (13,14-dihydro-15-keto-PGF2α) in pregnant and pseudo-pregnant Iberian lynx: A new noninvasive pregnancy marker for felid species
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DOI：
10.1016/j.theriogenology.2009.10.008
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发表时间：
2010-03-01
期刊：
影响因子：
2.8
通讯作者：
Dehnhard, M.
中科院分区：
文献类型：
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作者：
Finkenwirth, C.;Jewgenow, K.;Dehnhard, M.
在哺乳动物中，子宫和胎盘前列腺素 F-2 α 参与生殖相关过程的调节，例如胚胎发育、分娩起始和卵巢活动恢复。前列腺素 F-2 α (PGF(2 α)) 快速代谢为其血浆代谢物 PGFM (13,14-二氢-15-酮-PGF(2 α))，在尿液中也可检测到。因此，本研究旨在开发和验证一种高效、快速且廉价的酶免疫分析 (EIA)，用于评估伊比利亚山猫 (Lynx pardinus) 尿液中的 PGFM，用于妊娠监测以及区分妊娠和假妊娠。从圈养的伊比利亚山猫（11 个怀孕周期和 4 个假怀孕周期）收集的尿液样本直接进行 PGFM EIA。该检测方法经过尿液 PGFM 的平行性、精确度和稳定性验证。此外，还进行了高效液相色谱 (HPLC) 免疫图和液相色谱质谱 (LCMS) 来鉴定尿液样本中的 PGFM。交配前和分娩后尿液 PGFM 水平约为 1.5 ng/mL。交配后第20天，怀孕和假怀孕的雌性激素水平均略有增加；在假孕女性中，这一升高不超过 7 ng/mL。交配后第 45 天观察到怀孕雌性的数量显着增加；尿 PGFM 从第 45 天的 10 ng/mL 增加到分娩前后的峰值 46.0 +/- 19.3 ng/mL。初步结果表明，PGFM 在粪便中也可检测到，并且遵循与尿液中所示类似的过程。总之，如果在分娩前约 20 天应用纯尿或粪便提取物，所提出并经过验证的 PGFM 测定是伊比利亚山猫（可能还有其他猫科动物）无创妊娠诊断的一种简单可靠的方法。尿液或粪便样本中的高 PGFM 水平可以在不了解交配时间的情况下进行妊娠诊断，从而使 PGFM 测试适用于自由放养的动物。 (C) 2010 Elsevier Inc. 保留所有权利。
In mammals, uterine and placental prostaglandin F-2 alpha is involved in the regulation of reproduction-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. Prostaglandin F-2 alpha (PGF(2 alpha)) is rapidly metabolized to its plasma metabolite PGFM (13,14-dihydro-15-keto-PGF(2 alpha)), which has also been detected in urine. Therefore, the current study aimed to develop and validate an efficient, quick, and inexpensive enzyme immunoassay (EIA) for PGFM estimation in urine of the Iberian lynx (Lynx pardinus) for pregnancy monitoring and for differentiation between pregnancy and pseudopregnancy. Urine samples collected from captive Iberian lynx (11 pregnant and 4 pseudo-pregnant cycles) were subjected directly to a PGFM EIA. The assay was validated for parallelism, precision, and stability of urinary PGFM. In addition, high-performance liquid chromatography (HPLC) immunograms and liquid chromatography mass spectrometry (LCMS) were performed to identify PGFM within urine samples. Urinary PGFM levels before mating and after parturition were about 1.5 ng/mL. After Day 20 postmating, both pregnant and pseudo-pregnant females showed slight increase of hormone levels; in pseudo-pregnant females, this elevation did not exceed 7 ng/mL. A significant increase in pregnant females was observed after Day 45 postmating; urinary PGFM increased from 10 ng/mL at Day 45 toward a peak of 46.0 +/- 19.3 ng/mL around parturition. First results show that PGFM is detectable in feces as well and follows similar courses as shown for urine. In conclusion, the presented and validated PGFM assay is an easy and reliable method for noninvasive pregnancy diagnosis in the Iberian lynx (and probably other felids) if applied approximately 20 d prior parturition in pure urine or fecal extracts. High PGFM levels in urine or fecal samples may allow a pregnancy diagnosis without knowledge of mating time, making the PGFM test applicable to free-ranging animals. (C) 2010 Elsevier Inc. All rights reserved.