Augmentations of glucose uptake and glucose transporter-1 in macrophages following thermal injury and sepsis in mice
Augmentations of glucose uptake and glucose transporter-1 in macrophages following thermal injury and sepsis in mice
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DOI：
10.1002/jlb.59.5.639
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发表时间：
1996-05-01
影响因子：
5.5
通讯作者：
Hofmann, CA
中科院分区：
文献类型：
--
作者：
Gamelli, RL;Liu, H;Hofmann, CA
葡萄糖是主要代谢产物：巨噬细胞的底物，其是宿主对损伤和感染的反应的关键组分。我们已经进行了一系列研究以在mRNA和蛋白质水平上检测巨噬细胞葡萄糖摄取和葡萄糖转运蛋白1（GLUT 1）的状态，从经历假烧伤（S）的小鼠获得的腹膜巨噬细胞，15% TBSA烧伤（B）+/-绿脓杆菌烧伤感染（B + I）和脂多糖（LPS）或肿瘤坏死因子-α（TNF-α）给药后，[H-3]脱氧葡萄糖摄取显著增加，（B组，157 +/- 9%; B + I组，243 +/- 19%; S + LPS组，231 +/- 24%; S + TNF-α组，379 +/- 18%; B + LPS组，230 +/- 13%;和B + TNF组，305 +/-23%，P < 0.01 vs，假手术组）。GLUT 1 mRNA和蛋白水平也增加（mRNA：B，135 +/- 13%; B + I，250 +/- 33%; S + LPS，282 +/- 29%; S + TNF-α，193 +/- 19%; B + LPS，378 +/- 20%;和B + TNF-α，204 +/- 16%;蛋白质：B，159 +/- 27%; B + I，181 +/- 17%; S + LPS，219 +/- 26%; S + TNF-α，343 +/- 51%; B + LPS，366 +/- 41%;和B + TNF-α，415 +/- 44，P < 0.01，与假手术相比），在体外与LPS或TNF-α共培养的巨噬细胞表现出类似的反应模式。巨噬细胞增加其细胞葡萄糖摄取，这是由增加的GLUT 1 mRNA和蛋白质水平促进的，由LPS引发的TNF-α可以在葡萄糖进入细胞的点处介导这种增强的碳水化合物代谢。
Glucose is the primary metabolic: substrate of macrophages, which are critical components of the host response to injury and infection, We have carried out a series of studies to examine macrophage glucose uptake and the status of glucose transporter 1 (GLUT1) at both the mRNA and protein level, Peritoneal macrophages that were obtained from mice undergoing sham burned (S), 15% TBSA burn (B) +/- Pseudomonas aeruginosa burn infection (B + I) and lipopolysaccharide (LPS) or tumor necrosis factor-alpha (TNF-alpha) administration, [H-3]deoxyglucose uptake was significantly increased (B, 157 +/- 9%; B + I, 243 +/- 19%; S + LPS, 231 +/- 24%; S + TNF-alpha, 379 +/- 18%; B + LPS, 230 +/- 13%; and B + TNF, 305 +/- 23%, P < 0.01 vs, sham). GLUT1 mRNA and protein levels were increased as well (mRNA: B, 135 +/- 13%; B + I, 250 +/- 33%; S + LPS, 282 +/- 29%; S + TNF-alpha, 193 +/- 19%; B + LPS, 378 +/- 20%; and B + TNF-alpha, 204 +/- 16%; protein: B, 159 +/- 27%; B + I, 181 +/- 17%; S + LPS, 219 +/- 26%; S + TNF-alpha, 343 +/- 51%; B + LPS, 366 +/- 41%; and B + TNF-alpha, 415 +/- 44, P < 0.01 vs, sham), Macrophages co-cultured with LPS or TNF-alpha in vitro demonstrated a similar response pattern, Following burn injury and infection, macrophages augment their cellular glucose uptake, which is facilitated by an increased GLUT1 mRNA and protein levels, TNF-alpha elicited by LPS may mediate this enhanced carbohydrate metabolism at the point of glucose entry into the cell.