Polydopamine coated magnetic-chitin (MCT) particles as a new matrix for enzyme immobilization
Polydopamine coated magnetic-chitin (MCT) particles as a new matrix for enzyme immobilization
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DOI：
10.1016/j.carbpol.2010.03.036
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发表时间：
2011-03
影响因子：
11.2
通讯作者：
M. Sureshkumar;Cheng-Kang Lee
中科院分区：
文献类型：
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作者：
M. Sureshkumar;Cheng-Kang Lee
采用甲壳素作为共沉淀法制备磁性颗粒的保护性和分散性基质。采用多巴胺对磁性甲壳素进行修饰，制备出平均粒径约1.5μm的磁性甲壳素颗粒，作为一种有效的酶固定化基质。多巴胺被自聚合并包覆在MCT上，为酶提供了黏附的表面。α-淀粉酶是一种淀粉水解酶，可以很容易地固定在多巴胺包被的微球上。戊二醛处理可进一步提高DMCT颗粒的固定化效率。表面固定化α-淀粉酶的淀粉水解率与游离酶相当。然而，在较宽的pH和温度范围内，酶的相对活性高于游离酶。磁性可回收固定化α-淀粉酶在重复使用6次后仍保持原有活力的70%以上。
Chitin was used as a protective and dispersive matrix for the preparation of magnetic granules in the co-precipitation process. The easily prepared magnetic-chitin (MCT) particles with average size about 1.5μm were modified with dopamine to be an effective enzyme immobilization matrix. Dopamine was self-polymerized and coated onto MCT, offering adherent surface for enzymes. A starch hydrolysis enzyme, α-amylase can be easily immobilized on the polydopamine coated MCT (DMCT). Glutaraldehyde treatment can further enhance the enzyme immobilization efficiency on DMCT particles. The surface immobilized α-amylase demonstrated a comparable starch hydrolysis rate as the free enzyme. However, the relative activities are greater than free enzyme over wider pH and temperature ranges. The magnetically retrievable immobilized α-amylase retained over 70% of its original activity after six times of repeated use.