DNA-catalyzed reactivity of a phosphoramidate functional group and formation of an unusual pyrophosphoramidate linkage.
DNA-catalyzed reactivity of a phosphoramidate functional group and formation of an unusual pyrophosphoramidate linkage.
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DNA 催化的氨基磷酸酯官能团的反应性和不寻常的焦磷酸氨基酯键的形成。
DOI：
10.1039/c1ob06088k
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发表时间：
2012
影响因子：
3.2
通讯作者：
Silverman,ScottK
中科院分区：
文献类型：
--
作者：
Sachdeva,Amit;Silverman,ScottK
在DNA催化的赖氨酸反应性的体外筛选中，我们发现了一种脱氧核酶，它催化5‘-三磷酸-RNA上的磷酰胺官能团的亲核攻击，形成不寻常的焦磷酰胺(N-PV-O-PV)键。这一发现突显了使用核酸催化剂的氮的亲核性相对较差，这表明未来的实验研究面临重大挑战。
During in vitro selection for DNA-catalyzed lysine reactivity, we identified a deoxyribozyme that instead catalyzes nucleophilic attack of a phosphoramidate functional group at a 5′-triphosphate-RNA, forming an unusual pyrophosphoramidate (N–PV–O–PV) linkage. This finding highlights the relatively poor nucleophilicity of nitrogen using nucleic acid catalysts, indicating a major challenge for future experimental investigation.