ENHANCEMENT OF DENGUE VIRUS-INFECTION IN MONOCYTES BY FLAVIVIRUS ANTISERA
ENHANCEMENT OF DENGUE VIRUS-INFECTION IN MONOCYTES BY FLAVIVIRUS ANTISERA
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DOI：
10.4269/ajtmh.1980.29.638
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发表时间：
1980-01-01
影响因子：
3.3
通讯作者：
OROURKE, EJ
中科院分区：
文献类型：
--
作者：
HALSTEAD, SB;PORTERFIELD, JS;OROURKE, EJ
在人外周血单核吞噬细胞（PBL）悬浮培养物中，通过亚中和浓度的登革抗血清产生的登革2型病毒（D2V）感染增强已经有报道。本研究发现，增强现象是代表性黄病毒抗血清的一个普遍特性。在家兔中1 ~ 3次黄病毒培养的24种抗血清中，除1种抗血清外，其余抗血清均能促进登革2型病毒在人PBL中的生长。在血凝抑制试验中，黄病毒抗血清对42种黄病毒抗原表现出最高水平的交叉反应性，在PBL培养中增强登革热复制最有效。交叉中和反应性与D2V感染增强无关。近1/2的黄病毒抗血清在1:10或1:20的稀释下能中和D2V。异型D2V中和抗体可以抑制体内感染的增强。应在实地观察中寻找可能增强异型黄病毒免疫的登革热感染。
Enhanced dengue 2 virus (D2V) infection in suspension cultures of human peripheral blood mononuclear phagocytes (PBL) produced by subneutralizing concentrations of dengue antisera was described previously. In this study, the enhancement phenomenon was found to be a general property of representative flavivirus antisera. All except 1 of 24 antisera, which were raised by 1-3 injections of flaviviruses in rabbits, enhanced the growth of dengue 2 virus in human PBL. Flavivirus antisera showing the greatest level of cross-reactivity against a battery of 42 flavivirus antigens in the hemagglutination-inhibition test were most potent in enhancing dengue replication in PBL cultures. Cross-neutralizing reactivity did not relate to enhanced D2V infection. Nearly 1/2 of studied flavivirus antisera neutralized D2V at dilutions of 1:10 or 1:20. Heterotypic D2V neutralizing antibody could serve as a brake on infection enhancement in vivo. Observations should be made in the field to look for possible enhancement of dengue infection in heterotypic flavivirus immunes.