Puffer smells tetrodotoxin
Puffer smells tetrodotoxin
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DOI：
10.1007/s10228-013-0353-z
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发表时间：
2013-11-01
影响因子：
1.2
通讯作者：
Sakakura, Yoshitaka
中科院分区：
文献类型：
--
作者：
Okita, Kogen;Yamazaki, Hideki;Sakakura, Yoshitaka
采用Y型迷宫对红鳍东方鲀进行了行为学观察，以探讨嗅觉对河豚毒素（TTX）检测的作用。我们将琼脂糖载体或一个琼脂糖和一个含有TTX（200 MU）的琼脂糖放置在Y-迷宫通道的每个头部。然后将三只无毒的孵化器饲养的幼鱼（体长，5.6 +/-A0.4cm; n = 18）释放到Y-迷宫中，并观察对载体的啄食行为3小时。同样的程序进行了测试嗅觉消融的青少年和青少年接受假手术。少年表现出显着的选择性TTX，除了嗅觉消融的少年。这些结果表明河豚鱼通过嗅觉器官检测TTX。
Behavioral observation was conducted to test whether olfaction is functional to detect tetrodotoxin (TTX) in tiger puffer Takifugu rubripes using Y-maze. We placed either agarose carrier or one agarose and one agarose containing TTX (200 MU) at each head of the channel of Y-maze. Then three non-toxic hatchery-reared juveniles (body length, 5.6 +/- A 0.4 cm; n = 18) were released into the Y-maze and pecking behavior to carrier was observed for 3 h. The same procedure was tested for olfactory-ablated juveniles and for juveniles taht received sham operation. Juveniles showed significant selectivity to TTX, except for olfactory-ablated juveniles. These results indicate that pufferfish detects TTX by olfactory organ.