Drosophila blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway
Drosophila blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway
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DOI：
10.1128/mcb.01304-07
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发表时间：
2007-12-01
影响因子：
5.3
通讯作者：
Ueda, Hitoshi
中科院分区：
文献类型：
--
作者：
Agawa, Yasuo;Sarhan, Moustafa;Ueda, Hitoshi
控制发育事件发生时间的调节机制对于正常发育的发生至关重要。ftz-fl在蜕皮激素脉冲后以时间调节的方式表达，并且这种精确表达对于黑腹果蝇的发育是必需的。为了了解昆虫激素蜕皮甾体如何调节FTZ-F1表达的时间，我们纯化了FTZ-F1的DNA结合调节剂。质谱分析显示，该蛋白质是哺乳动物B淋巴细胞诱导成熟蛋白1（Blimp-1）的果蝇同源物。果蝇Blimp-1（dBlimp-1）是由20-羟基蜕皮激素直接诱导的，其产物存在于高蜕皮激素时期，并迅速翻转。dBlimp-1的强制表达和RNA干扰分析表明，dBlimp-1充当抑制子并控制FTZ-F1表达的时机。此外，其延长的表达导致化蛹时间延迟。这些结果表明，瞬时转录抑制因子dBlimp-1是重要的蜕皮激素诱导的途径，以确定发育时间。
Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-fl is expressed in a temporally regulated manner following pulses of ecdysteroid and this precise expression is necessary for the development of Drosophila melanogaster. To understand how insect hormone ecdysteroids regulate the timing of FTZ-F1 expression, we purified a DNA binding regulator of ftz-fl. Mass spectroscopy analysis revealed this protein to be a fly homolog of mammalian B lymphocyte-induced maturation protein 1 (Blimp-1). Drosophila Blimp-1 (dBlimp-1) is induced directly by 20-hydroxyecdysone, and its product exists during high-ecdysteroid periods and turns over rapidly. Forced expression of dBlimp-1 and RNA interference analysis indicate that dBlimp-1 acts as a repressor and controls the timing of FTZ-F1 expression. Furthermore, its prolonged expression results in delay of pupation timing. These results suggest that the transient transcriptional repressor dBlimp-1 is important for determining developmental timing in the ecdysone-induced pathway.