Tetrahymena pyriformis infection in the tiger barb, Puntius tetrazona: Parasite characterization and pathology of infected fish
Tetrahymena pyriformis infection in the tiger barb, Puntius tetrazona: Parasite characterization and pathology of infected fish
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DOI：
10.1016/j.aquaculture.2021.737725
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发表时间：
2021-11
期刊：
影响因子：
4.5
通讯作者：
Xuming Pan;Wenyu Li;A. Warren;Zhongdian Dong
中科院分区：
文献类型：
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作者：
Xuming Pan;Wenyu Li;A. Warren;Zhongdian Dong
梨形四膜虫是一种组织食性原生动物纤毛虫，负责多种水生生物（包括虎倒刺Puntiustetrazona）的四膜虫病。T 的形态、结构、SSU rRNA 和cox 1 基因序列。梨形分离自 P.采用活体观察和银染法对哈尔滨某水产市场的四带藻进行了调查。此外，设计了一种用荧光染料标记的 SSU-rDNA 靶向寡核苷酸探针，用于快速识别 T。梨形。病鱼皮肤上分泌大量粘液，眼睛和尾鳍基部有深红色斑点。所有病鱼的鳃都枯萎、苍白。苏木精-伊红染色组织学检查可直接发现梨形菌。结果表明，一些T.梨状细胞存在于鳃丝的表面。腹部也出现异常的彩旗，鳃丝和肌肉组织变形、萎缩、破裂。成功感染P. tetrazonawas 是通过 T 实现的。通过用一定范围的感染剂量进行刮擦接种来防治梨形菌。结果表明，T.梨状肌侵入P.四带菌通过体表病变传播，大的感染剂量可加速受感染鱼的死亡。
Tetrahymena pyriformisis a histophagus protozoan ciliate responsible for tetrahymenosis in various aquatic organisms including the tiger barbPuntiustetrazona.The morphology, infraciliature, SSU rRNA andcox 1gene sequences ofT. pyriformisisolated fromP. tetrazonafrom a fish market in Harbin was investigated using living observation and silver staining methods. In addition, an SSU-rDNA-targeted oligonucleotide probe labelled with a fluorochrome was designed for rapid identification ofT. pyriformis. Diseased fish secreted copious amounts of mucus on the skin and had dark-red spots in the eyes and at the base of tail fins. Gills of all diseased fish were shriveled and pale.T. pyriformiscould be directly found when examined histologically by hematoxylin-eosin staining. The results showed that someT. pyriformiscells were present on the surface of the gill filaments. There was also abnormal bunting of the abdomen, and the gill filaments and muscle tissue were deformed, shrunken, and cracked. Successful infection ofP. tetrazonawas achieved withT. pyriformisby scratch inoculation with a range of infectious doses. The results indicate thatT. pyriformisinvadesP. tetrazonathrough lesions on the body surface, and a large infectious dose could accelerate the death of the infected fish.