Regulation of Iron on Bacterial Growth and Production of Thermostable Direct Hemolysin by Vibrio parahaemolyticus in Intraperitoneal Infected Mice
Regulation of Iron on Bacterial Growth and Production of Thermostable Direct Hemolysin by Vibrio parahaemolyticus in Intraperitoneal Infected Mice
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DOI：
10.1111/j.1348-0421.1994.tb01792.x
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发表时间：
1994-05
影响因子：
2.6
通讯作者：
H. Wong;Yeong‐sheng Lee
中科院分区：
文献类型：
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作者：
H. Wong;Yeong‐sheng Lee
副溶血性弧菌的发病机制尚不清楚。本文研究了铁对细菌增殖和耐热直接溶血素（TDH）产生的影响。在柠檬酸铁铵（100 μg/ml）存在下注射细菌培养物可显著增强小鼠的致死率，同时激活细菌在体内的增殖。铁限制的培养物显示出比那些富铁培养物更好的增殖，以响应补充铁源的添加。溶血菌株ST 550和D 62在铁限制培养物中产生的TDH高于富铁培养物。通过铁限制或富铁培养物的TDH的产生被铁的添加抑制。铁对副溶血性弧菌的毒力增强作用可能是通过激活细菌体内增殖而不是刺激TDH的产生。在铁限制条件下预培养的副溶血性弧菌可能更适应体内环境。
Pathogenesis of Vibrio parahaemolyticus is not clearly understood. Effects of iron on the bacterial proliferation and production of thermostable direct hemolysin (TDH) in intraperitoneal infected mice were studied. Injection of bacterial culture in the presence of ferric ammonium citrate (100 μg/ml) significantly enhanced the lethality for mice, and simultaneously activated bacterial proliferation in vivo. The iron‐limited cultures showed better proliferation than those iron‐rich cultures in response to the addition of supplementary iron source. Production of TDH by the hemolytic strains ST550 and D62 was higher in the iron‐limited cultures than the iron‐rich cultures. Production of TDH by both the iron‐limited or iron‐rich cultures was inhibited by the addition of iron. In conclusion, the virulence enhancement effect of iron in V. parahaemolyticus was probably by activating bacterial proliferation in vivo and not by stimulating the production of TDH. V. parahaemolyticus precultured in iron‐limited condition may be more adaptable to in vivo environment.