Methanol Extract of Cassia mimosoides var. nomame Attenuates Myocardial Injury by Inhibition of Apoptosis in a Rat Model of Ischemia-Reperfusion.
Methanol Extract of Cassia mimosoides var. nomame Attenuates Myocardial Injury by Inhibition of Apoptosis in a Rat Model of Ischemia-Reperfusion.
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DOI：
10.3746/pnf.2012.17.3.177
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发表时间：
2012-09
影响因子：
--
通讯作者：
Lee J
中科院分区：
文献类型：
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作者：
Lim SH;Lee J
通过冠状动脉的血流中断及其随后的恢复触发了活性氧簇（ROS）的爆发，导致心肌细胞死亡。在这项研究中，我们确定了含羞草的甲醇提取物是否。野芝麻对心肌缺血再灌注损伤有保护作用。用α，α-二苯基-β-苦肼基（DPPH）自由基猝灭法测定其体外自由基清除活性，结果表明其活性略低于抗坏血酸。对大鼠口服该提取物（400 mg/kg/天）3天后，通过结扎冠状动脉左前降支（LAD）30分钟，然后再灌注3小时，产生心肌缺血/再灌注损伤。与载体处理组相比，施用提取物使提取物处理组中的梗死面积（IS）（梗死面积与危险面积的比率）显著降低28.3%。Bax/Bcl-2比值降低33.3%，caspase-3活化抑制40%，随后凋亡细胞数减少66.3%，从而减轻了细胞损伤。这些结果表明，该提取物通过清除ROS（包括自由基）并因此阻断凋亡级联反应来减轻缺血-再灌注大鼠模型中的心肌损伤。因此，摄入决明子mimosoides变种。野芝麻对预防缺血性心肌损伤有一定的作用。
Interruption of blood flow through coronary arteries and its subsequent restoration triggers the generation of a burst of reactive oxygen species (ROS), leading to myocardial cell death. In this study, we determined whether a methanol extract of Cassia mimosoides var. nomame Makino could prevent myocardial ischemia-reperfusion injury. When radical scavenging activity of the extract was measured in vitro using its α,α-diphenyl-β-picrylhydrazyl (DPPH) radical quenching ability, the extract showed an activity slightly lower than that of ascorbic acid. Three days after oral administration of the extract (400 mg/kg/day) to rats, myocardial ischemia/reperfusion injury was generated by 30 min of ligation of the left anterior descending coronary artery (LAD), followed by 3 hr reperfusion. Compared with the vehicle-treated group, administration of the extract significantly reduced infarct size (IS) (ratio of infarct area to area at risk) in the extract-treated group by 28.3%. Reduction in the cellular injury was mediated by attenuation of Bax/Bcl-2 ratio by 33.3%, inhibition of caspase-3 activation from procas-pase-3 by 40%, and subsequent reduction in the number of apoptotic cells by 66.3%. These results suggest that the extract attenuates myocardial injury in a rat model of ischemia-reperfusion by scavenging ROS, including free radicals, and consequently blocking apoptotic cascades. Therefore, intake of Cassia mimosoides var. nomame Makino might be beneficial for preventing ischemic myocardial injury.