GAR1 IS AN ESSENTIAL SMALL NUCLEOLAR RNP PROTEIN REQUIRED FOR PRE-RIBOSOMAL-RNA PROCESSING IN YEAST
GAR1 IS AN ESSENTIAL SMALL NUCLEOLAR RNP PROTEIN REQUIRED FOR PRE-RIBOSOMAL-RNA PROCESSING IN YEAST
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DOI：
10.1002/j.1460-2075.1992.tb05099.x
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发表时间：
1992-02-01
期刊：
影响因子：
11.4
通讯作者：
LAPEYRE, B
中科院分区：
文献类型：
--
作者：
GIRARD, JP;LEHTONEN, H;LAPEYRE, B
在少数已被鉴定的真核核仁蛋白中，核仁蛋白、纤原蛋白、SSB1和NSR1具有一个共同的结构基序GAR结构域，该结构域富含甘氨酸和精氨酸残基。为了研究这个结构域的存在是否是核仁蛋白家族的特征，我们研究了其他酵母基因是否编码含有GAR结构域的蛋白质。我们在此报告了一个新的酵母基因GAR1的序列和特性，该基因编码含有两个GAR结构域的205个残基的蛋白质。GAR1是一种非核糖体蛋白，定位于酵母核仁，对细胞生长至关重要。抗GAR1抗体的免疫沉淀显示GAR1与snR10和snR30等snorna亚群相关。在受调控的GAL启动子的控制下表达GAR1，会损害pre-rRNA 35S初级转录本的加工，并阻止18S rRNA的合成。因此，GAR1是含有GAR结构域的核仁蛋白家族的第五个成员，并参与rRNA代谢。
Among the few proteins of the eukaryotic nucleolus that have been characterized, four proteins, nucleolin, fibrillarin, SSB1 and NSR1, possess a common structural motif, the GAR domain, which is rich in glycine and arginine residues. In order to examine whether the presence of this domain is characteristic of a family of nucleolar proteins, we investigated whether other yeast genes encode proteins containing GAR domains. We report here the sequence and the characterization of a new yeast gene, GAR1, which encodes a protein of 205 residues containing two GAR domains. GAR1 is a nonribosomal protein, localized in the yeast nucleolus, which is essential for cell growth. Immunoprecipitation with anti-GAR1 antibodies shows that GAR1 is associated with a subset of snoRNAs, including snR10 and snR30. Depletion of GAR1 by expression under the control of a regulated GAL promoter, impairs processing of the 35S primary transcript of pre-rRNA and prevents synthesis of 18S rRNA. GAR1 is thus the fifth member of a family of nucleolar proteins containing GAR domains, and is involved in rRNA metabolism.